Project description:Pseudomonas fluorescens FW300-N2C3 was cultured in succinate minimal media (SMM) and analyzed for metabolites on LCMS with iron infusions of either 2 mM or 500 micromolar iron.
Project description:Ensifer meliloti strains USDA 1021 and USDA 1157 were cultured in succinate minimal media and analyzed for metabolites on LCMS. Analyze extracted with SPE using 80% or 100% methanol.
Project description:Ensifer meliloti strains USDA 1021 and USDA 1157 were cultured in succinate minimal media (SMM) and then analyzed for metabolites with LCMS and an iron infusion.
Project description:Ensifer meliloti strains USDA 1021 and USDA 1157 were cultured in succinate minimal media (SMM) with and without 10 micromolar FeCl3. Samples were analyzed for metabolites on LCMS.
Project description:Histidine is a good source of nutrient for many bacteria, but its utilization poses a significant challenge as it delivers excess nitrogen over carbon. The rate of histidine catabolism (hut) must thus be tightly regulated. Here, in Pseudomonas fluorescens SBW25, we show that the CbrAB two-component system directly activates the transcription of hut genes, while mediating succinate-induced carbon catabolite repression of hut at the translational level via the CbrAB-CrcYZ-Hfq/Crc regulatory cascade. When growing in minimal salt medium supplemented with histidine and succinate (the most preferred carbon source), the CbrAB-mediated promoter activity is weak; and the global nitrogen regulator NtrBC plays the dominant role in directly activating hut transcription.
Project description:Transcriptomic profiling of Pseudomonas fluorescens Pf-5 comparing iron(II) chloride supplemented grown culture against non-iron treated grown culture in M9 minimal media Two-condition experiment, iron(II) chloride supplemented culture versus non-iron treated culture. 4 biological replicates including 3 technical replicates for one of the biological replicates. Swap-dye experiments were performed
Project description:Transcriptomic profiling of Pseudomonas fluorescens Pf-5 comparing culture treated with 160 µg/mL tannic acid against non-treated culture grown in Mueller-Hinton media
Project description:Transcriptomic profiling of Pseudomonas fluorescens Pf-5 comparing culture treated with 20 µg/mL tannic acid against non-treated culture grown in Mueller-Hinton media