GNPS - CMMC_Bile_Salt_Hydrolase_Rxn_5.1
Ontology highlight
ABSTRACT:
INSTRUMENT(S): Q Exactive
ORGANISM(S): No Species
SUBMITTER: 
Pieter C. Dorrestein
PROVIDER: MSV000094811 | GNPS | Mon May 20 16:10:00 BST 2024
REPOSITORIES: GNPS
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Project description:MS/MS fragmentation data of BSH enzyme assay of taurylcholic acid with a mix of 10 amino acids (Phenylalanine, Alanine, Arginine, Asparagine, Cysteine, Glutamine, Histidine, Methionine, Lysine, and Isoleucine) acquired on Q Exactive - with chromatographic separation on a Phenomenex polar C18 column.
2024-05-20 | MSV000094811 | MassIVE
Project description:MS/MS fragmentation data of BSH enzyme assay of cholic glycine with a mix of 10 amino acids (Phenylalanine, Alanine, Arginine, Asparagine, Aspartic Acid, Cysteine, Glutamic Acid, Glutamine, Histidine, and Isoleucine) acquired on Q Exactive - with chromatographic separation on a Phenomenex polar C18 column.
2024-04-04 | MSV000094468 | MassIVE
Project description:MS/MS fragmentation data of BSH enzyme assay of cholic glycine with a mix of 10 amino acids (Phenylalanine, Alanine, Arginine, Asparagine, Aspartic Acid, Cysteine, Glutamic Acid, Glutamine, Histidine, and Isoleucine) acquired on Q Exactive - with chromatographic separation on a Phenomenex polar C18 column.
2024-05-07 | MSV000094707 | MassIVE
Project description:MS/MS fragmentation data of BSH enzyme assay of taurylcholic acid with a mix of 10 amino acids (Phenylalanine, Alanine, Arginine, Asparagine, Cysteine, Glutamine, Histidine, Methionine, Lysine, and Isoleucine) acquired on Q Exactive - with chromatographic separation on a Phenomenex polar C18 column.
2024-05-17 | MSV000094789 | GNPS
Project description:MS/MS fragmentation data of BSH enzyme assay of cholic glycine with a mix of 10 amino acids (Phenylalanine, Alanine, Arginine, Asparagine, Aspartic Acid, Cysteine, Glutamic Acid, Glutamine, Histidine, and Isoleucine) acquired on Q Exactive - with chromatographic separation on a Phenomenex polar C18 column.
2024-05-07 | MSV000094707 | GNPS
Project description:MS/MS fragmentation data of BSH enzyme assay of cholic glycine with a mix of 10 amino acids (Phenylalanine, Alanine, Arginine, Asparagine, Aspartic Acid, Cysteine, Glutamic Acid, Glutamine, Histidine, and Isoleucine) acquired on Q Exactive - with chromatographic separation on a Phenomenex polar C18 column.
2024-04-04 | MSV000094468 | GNPS
Project description:MS/MS fragmentation data of 2 BSH enzyme assays. One with taurylcholic acid and the other with glycocholic acid each with a mix of 43 amino compounds (Asparagine, Aspartic Acid, L-Glutamic Acid, Glutamine, Methionine, 4-aminophenol, Tryptamine, B-Alanine, L-Carnosine, Allantoin, 2-Phenylglycine, L-aspartyl-L-phenylalanine methyl ester, Isoniazid, DL-Proline, Creatine, Histamine. L-Alanine, L-Arginine, L-Cysteine, L-Leucine, Lysine, Phenylalanine, Serine, Tryptophan, Valine, 1,3-Diaminopropane, L-citrulline, 5-Aminovaleric Acid, Dopamine, Serotonin, Histidine, L-Homoserine, L(+)-Ornithine, gamma-Aminobutyric acid, L-Glutathione (oxidized form), trans-4-Hydroxy-L-proline, L(+)-Homoarginine, Glycyl-L-Valine, L-2-Aminoadipic Acid, L-Glutathione reduced, 3-methoxytyramine, Threonine, and Tyramine) acquired on Q Exactive - with chromatographic separation on a Phenomenex polar C18 column.
2024-07-02 | MSV000095220 | GNPS
Project description:RNA was isolated from early stationary phase S. gordonii V288(OD620 nm ~ 1.8).Bacteria were grown in chemically defined synthetic media (FMC) ( K2HPO4, 300 µg ml-1; KH2PO4 440 µg ml-1; Na2HPO4, 3.15 mg ml-1; NaH2PO4, 2.05 mg ml-1; (NH4)2SO4 0.6 mg ml-1; sodium citrate, 225 µg ml-1; riboflavin, 0.4 µg ml-1; biotin, 0.01 µg ml-1; folic acid, 0.1 µg ml-1; pantothenate, 0.8 µg ml-1; p-aminobenzoic acid 0.1 µg ml-1; thiamine 0.4 µg ml-1; nicotinamide 2.0 µg ml-1; pyridoxamine 0.8 µg ml-1; D-glucose 20 mg ml-1; sodium acetate 6 mg ml-1; adenine 35 µg ml-1; guanine µg ml-1; uracil µg ml-1; MgSO4, 200 µg ml-1; NaCl, 10 µg ml-1; FeSO4, 10 µg ml-1; MnSO4 10 µg ml-1; glutamine 5 µg ml-1; L-glutamic acid, 300 µg ml-1; L-valine, 100 µg ml-1; 110 µg ml-1 of L-lysine, L-asparate, L-isoleucine, L-methionine, L-serine, L-phenylalanine and 200 µg ml-1 of L-alanine, L-cystine, L-histidine, glycine, L-hydroxyproline, L-proline, L-tryptophan and L-tyrosine, pH 6.5) (Terleckyj & Shockman, 1975; Terleckyj et al., 1975) at 37°C in 5% CO2.
2008-08-01 | E-MEXP-1712 | biostudies-arrayexpress
Project description:Objective: Insulin regulates amino acid metabolism. We investigated whether glycemia and 43 genetic risk variants for hyperglycemia/type 2 diabetes affect amino acid levels in a large population-based cohort. Subjects and Methods: A total of 9,371 non-diabetic or newly-diagnosed type 2 diabetic Finnish men from the population-based METSIM Study were studied. Proton NMR spectroscopy was used to measure plasma levels of 8 amino acids. Genotyping of 42 SNPs and mRNA microarray analysis from 200 subcutaneous adipose tissue samples were performed. Results: Increasing fasting and/or 2-hour plasma glucose levels were associated with increasing levels of alanine, valine, leucine, isoleucine, phenylalanine and tyrosine, and decreasing levels of histidine and glutamine. We also found significant correlations between insulin sensitivity (Matsuda ISI) and expression of genes regulating amino acid metabolism. Only one SNP (rs780094 in GCKR) of the 42 risk SNPs for type 2 diabetes or hyperglycemia was significantly associated with the levels of alanine, isoleucine, and glutamine. Conclusions : We observed that the levels of branched-chain, aromatic amino acids and alanine increased and the levels of glutamine and histidine decreased with increasing glycemia. These associations seemed to be mediated by insulin resistance, at least in part. GCKR rs780094 was significantly associated with several amino acids. Total RNA was obtained from subcutaneous fat biopsies from 200 people participating in the METSIM study (4 samples were replicated for a total of 204 arrays).
2012-04-01 | E-GEOD-32512 | biostudies-arrayexpress
Project description:Objective: Insulin regulates amino acid metabolism. We investigated whether glycemia and 43 genetic risk variants for hyperglycemia/type 2 diabetes affect amino acid levels in a large population-based cohort. Subjects and Methods: A total of 9,371 non-diabetic or newly-diagnosed type 2 diabetic Finnish men from the population-based METSIM Study were studied. Proton NMR spectroscopy was used to measure plasma levels of 8 amino acids. Genotyping of 42 SNPs and mRNA microarray analysis from 200 subcutaneous adipose tissue samples were performed. Results: Increasing fasting and/or 2-hour plasma glucose levels were associated with increasing levels of alanine, valine, leucine, isoleucine, phenylalanine and tyrosine, and decreasing levels of histidine and glutamine. We also found significant correlations between insulin sensitivity (Matsuda ISI) and expression of genes regulating amino acid metabolism. Only one SNP (rs780094 in GCKR) of the 42 risk SNPs for type 2 diabetes or hyperglycemia was significantly associated with the levels of alanine, isoleucine, and glutamine. Conclusions : We observed that the levels of branched-chain, aromatic amino acids and alanine increased and the levels of glutamine and histidine decreased with increasing glycemia. These associations seemed to be mediated by insulin resistance, at least in part. GCKR rs780094 was significantly associated with several amino acids.
2012-04-02 | GSE32512 | GEO