Project description:Interventions: Natural Orifice Specimen Extraction:NOSE
Primary outcome(s): assessment of abdominal pain
Study Design: Single arm Non-randomized
Project description:In the present study, we analyzed single-cell multi-omics data from psoriasis patients and healthy individuals and found that more fibroblast-macrophage communication was present in the dermis of psoriasis lesions, exacerbating psoriasis progression. A natural product library was used to screen for a small molecule compound, celastrol, that could interfere with fibroblast-macrophage communication. It was demonstrated that celastrol targeted low-denisity lipoprotein receptor-related protein 1 (LRP1) to inhibit fibroblast secretion of CCL2 and inhibited psoriasis progression by reducing its recruitment to macrophages, thereby blocking communication between the two cells. Moreover, conditional knockdown of LRP1 by fibroblasts significantly improved psoriasis in mice, suggesting that LRP1 may be an important target for the treatment of psoriasis.
Project description:Proteins identified from zooarchaeological turkey (Meleagris gallopavo), rabbit (Lagomorpha), and squirrel (Sciuridae) remains using non-targeted LC-MS/MS. Development of potential extraction and characterization method for the improvement of LC-MS analysis of protein residues from prehistoric cooking pottery.
Project description:Gedunin is a natural product that affects LNCaP gene expression by 6h; We used microarrays to detail the global programme of gene expression affected by gedunin treatment at 6h Experiment Overall Design: LNCaP cells were grown to 50% confluency and treated with gedunin for 6h prior to direct Trizol lysis and RNA isolation