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GNPS - Linking Ecology, Behavior, and Immunology to Spatio-Temporal Variation in Helminth Transmission - PRJ5

ABSTRACT: Samples of livers from stickleback fish captured from 47 lakes on Vancouver Island, with associated data on parasite infection status, immune traits, diet, and morphology. Untargeted LC-MS/MS acquisition was performed on a Vanquish Flex UHPLC system coupled to an Orbitrap Exploris 240 (Thermo Fisher Scientific, Bremen, Germany). Chromatographic separation was performed on a Kinetex 2.6 um 100 A pore size Polar C18 reversed phase UHPLC column 100 x 2.1 mm (Phenomenex, Torrance, CA).

INSTRUMENT(S): Orbitrap Exploris 240

ORGANISM(S): Gasterosteus Aculeatus (ncbitaxon:69293)

SUBMITTER: Daniel Bolnick

PROVIDER: MSV000095784 | GNPS |

REPOSITORIES: GNPS

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GNPS - Bolnick Alaska Experimental Evolution (2024) - PRJ7

Project description:Livers preserved in Ethanol from threespine stickleback (Gasterosteus aculeatus). Thirty per lake from 15 lakes in Alaska. Seven of the lakes are native populations, and eight of the lakes are experimentally introduced populations founded in 2019. Samples were collected by Dan Bolnick and colleagues in 2024. We are tracking evolution of stickleback as the newly founded populations adapt to their lake environments. Untargeted LC-MS/MS acquisition was performed on a Vanquish Flex UHPLC system coupled to an Orbitrap Exploris 240 (Thermo Fisher Scientific, Bremen, Germany). Chromatographic separation was performed on a Kinetex 2.6 um 100 A pore size Polar C18 reversed phase UHPLC column 100 x 2.1 mm (Phenomenex, Torrance, CA).

2024-08-07 | MSV000095540 | GNPS

GNPS - Fish-Tapeworm co-metabolomics (Fish Livers) - PRJ6_LIVERS

Project description:We have pairs of fish and their tapeworms, from Merril (MER), Vernon (VER), Nimpkish (NIM), and Woss (WOS) Lakes on Vancouver Island. The fish samples are livers, preserved in ethanol. The parasite samples are a third of each individual tapeworm. Untargeted LC-MS/MS acquisition was performed on a Vanquish Flex UHPLC system coupled to an Orbitrap Exploris 240 (Thermo Fisher Scientific, Bremen, Germany). Chromatographic separation was performed on a Kinetex 2.6 um 100 A pore size Polar C18 reversed phase UHPLC column 100 x 2.1 mm (Phenomenex, Torrance, CA).

| MSV000095773 | GNPS

Proteomic analysis of the secretome of Schistocephalus solidus LC-MS/MS

Project description:Schistocephalus solidus is a cestode parasite that is thought to manipulate the behaviour of its threespine stickleback host Gasterosteus aculeatus. It has been hypothesized that the worm could liberate in its external environment “manipulation factors” that would ultimately interfere with the host’s physiology and behaviour. The objective of this project is to describe the whole proteomic content of the proteome and of the secretome of a putative manipulative parasite, Schistocephalus solidus, with the aim to identify proteins that could be involved in the behavioural perturbations of the threespine stickleback.

2021-09-09 | PXD016844 | Pride

GNPS - Bolnick Alaska Experimental Evolution (2023) - PRJ1

Project description:Livers preserved in Ethanol from threespine stickleback (Gasterosteus aculeatus). Thirty per lake from 16 lakes in Alaska. Seven of the lakes are native populations, and nine of the lakes are experimentally introduced populations founded in 2019. Samples were collected by Dan Bolnick and colleagues in June 2023. Untargeted LC-MS/MS acquisition was performed on a Vanquish Flex UHPLC system coupled to an Orbitrap Exploris 240 (Thermo Fisher Scientific, Bremen, Germany). Chromatographic separation was performed on a Kinetex 2.6 um 100 A pore size Polar C18 reversed phase UHPLC column 100 x 2.1 mm (Phenomenex, Torrance, CA).

2024-08-27 | MSV000095708 | GNPS

Molecular analysis of endocrine disruption in hornyhead turbot captured from wastewater treatment plant discharge locations in Southern California

Project description:Hornyhead turbot (Pleuronichthys verticalis) captured near sewage outfalls are used as sentinel fish for monitoring exposure to industrial and agricultural chemicals of ~20 million people living in coastal southern California. Although analyses of hormones in blood and organ morphology and histology in fish are useful for assessing exposure, there is a need for quantitative and sensitive molecular measurements, as many contaminants produce subtle effects. A novel multispecies microarray and qRT-PCR were used to investigate endocrine disruption in turbot captured near sewage outfalls in San Diego, Orange County and Los Angeles California. Analysis of expression of genes involved in hormone [e.g. estrogen, androgen, thyroid] responses and xenobiotic metabolism in turbot livers was correlated with phenotypic end points.

2013-12-10 | E-MEXP-3852 | biostudies-arrayexpress

Project description:stickleback gut microbiota depended on parasite exposure

| PRJNA495340 | ENA

Transcriptomic responses of three-spined stickleback (Gasterosteus aculeatus) from Aire and Siblyback populations to ethinyl-estradiol, copper and binary mixtures

Project description:Sticklebacks were caught from the River Aire (Yorkshire, UK) and Siblyback reservoir (Cornwall, UK), depurated for 4 months at Exeter University, split into groups of 20 individuals before exposure. Fish were exposed to either control conditions, three concentrations of copper (3.2, 32, 128 Âµg/L; prepared using copper sulphate), three concentrations of ethinyl-estradiol (EE2) (1, 10, 32 ng/L) and three mixtures (3.2 Âµg Cu/L and 32 ng EE2/L; 128 Âµg Cu/L and 1 ng EE2/L; 128 Âµg Cu/L and 32 ng EE2/L) in duplicate tanks for 4 days. After sampling liver of male fish were stored at -80C for transcriptomics. Total RNA was prepared from fish livers, aliquots of all samples were pooled and labeled with Cy3-dCTP as a common reference, individual samples were labeled with Cy5-dCTP. Each hybridisation to the stickleback PGPS2 cDNA microarray consisited of one Cy5-labeled individual sample and one aliquot of the Cy3 labeled commmon reference sample. Data were captured with an Axon scanner using Genepix software.

2017-06-01 | E-MTAB-4907 | biostudies-arrayexpress

Standing genetic variation in Atlantic stickleback

Project description:Standing genetic variation in Atlantic stickleback

| PRJEB42736 | ENA

Microarray transcriptional profiling of three-spined stickleback (Gasterosteus aculeatus) exposed to 10 different model toxicants and 26 mixtures of these toxicants

Project description:Adult female three-spined stickleback fish (Gasterosteus aculeatus) were exposed to 10 individual chemicals, 26 mixtures of these chemicals, or control conditions in a flow-through system for 4 days. Transcriptomics was performed on liver samples by microarray. The main aims were to determine molecular signatures induced by these chemicals in the three-spined stickleback, discover whether these persisted in chemical mixtures and identify non-additive molecular responses in chemical mixtures exposures.

2018-03-01 | E-MTAB-5581 | biostudies-arrayexpress

Proteomics support the threespine stickleback egg coat as a protective oocyte envelope

Project description:After the end of the last ice age, ancestrally marine threespine stickleback fish (Gasterosteus aculeatus) have undergone an adaptive radiation into freshwater environments throughout the Northern Hemisphere, creating an excellent model system for studying molecular adaptation and speciation. Stickleback populations are reproductively isolated to varying degrees, despite the fact that they can be crossed in the lab to produce viable offspring. Ecological and behavioral factors have been suggested to underlie incipient stickleback speciation. However, reproductive proteins represent a previously unexplored driver of speciation. As mediators of gamete recognition during fertilization, reproductive proteins both create and maintain species boundaries. Gamete recognition proteins are also frequently found to be rapidly evolving, and their divergence may culminate in reproductive isolation and ultimately speciation. As an initial investigation into the contribution of reproductive proteins to stickleback reproductive isolation, we characterized the egg coat proteome of threespine stickleback eggs. In agreement with other teleosts, we find that stickleback egg coats are comprised of homologs to the zona pellucida (ZP) proteins ZP1 and ZP3. We explore aspects of stickleback ZP protein biology, including glycosylation, disulfide bonding, and sites of synthesis, and find many substantial differences compared to their mammalian homologs. Furthermore, molecular evolutionary analyses indicate that ZP3, but not ZP1, has experienced positive Darwinian selection across teleost fish. Taken together, these changes to stickleback ZP protein architecture suggest that the egg coats of stickleback fish, and perhaps fish more generally, have evolved to fulfill a more protective functional role than their mammalian counterparts.

2021-05-26 | PXD017489 | Pride

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