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GNPS - Microbiome Reciprocal Transfer Experiment 2023 - Investigating the effect on the host of `mismatched` microbiomes

ABSTRACT: Full reciprocal transfer of microbiomes between nine host strains of phytoplankton and microbe-free controls (Kuijpers et al unpublished). Microbiomes were naturally recruited from lake and stream water in an assembly experiment (Broe et al unpublished). Transferred microbiomes were added to axenic hosts and grown for 7 or 14 days before control or heat shock treatment.

INSTRUMENT(S): Orbitrap Exploris 240

ORGANISM(S): Porphyridium Aerugineum (ncbitaxon:2792) Chlorella Sorokiniana (ncbitaxon:3076) Chlorella Vulgaris (ncbitaxon:3077) Desmonostoc Muscorum (ncbitaxon:1179) Vischeria (ncbitaxon:44431) Chlamydomonas Reinhardtii (ncbitaxon:3055) Nannochloris (ncbitaxon:3187)

SUBMITTER: Sara Jackrel

PROVIDER: MSV000095905 | GNPS |

REPOSITORIES: GNPS

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Microbiome Reciprocal Transfer Experiment 2023 - Investigating the effect on the host of `mismatched` microbiomes

Project description:Full reciprocal transfer of microbiomes between nine host strains of phytoplankton and microbe-free controls (Kuijpers et al unpublished). Microbiomes were naturally recruited from lake and stream water in an assembly experiment (Broe et al unpublished). Transferred microbiomes were added to axenic hosts and grown for 7 or 14 days before control or heat shock treatment.

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Heat shock experimental replicates

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2016-12-13 | PXD004202 | Pride

Quantitative proteomics of cold adaptation of the Antarctic archaeon Halohasta litchfieldiae and Halorubrum lacusprofundi

Project description:Halohasta litchfieldiae and Halorubrum lacusprofundi are important members of Deep Lake in Antarctica, representing ~44% (the most abundance) and ~10% (the 3rd most abundance) of the lake population. Deep Lake is in the Vestfold Hills, East Antarctica (68°33’36.8S, 78°11’48.7E) and is 36 m deep, monomictic, and perennially cold (down to -20C) (DeMaere et al., 2013; Williams et al., 2014; Tschitschko et al., 2015; Tschitschko et al., 2016). In this project, using quantitative proteomics (8-plex iTRAQ labeling), proteins, pathways and cellular processes important for cold adaptation were determined for both Hht. litchfieldiae and Hrr. lacusprofundi. The data provided a new level of understanding about molecular mechanisms and regulatory networks involved in adaptation of the Antarctic haloarchaea to cold environment. DeMaere, M. Z., Williams, T. J., Allen, M. A., Brown, M. V., Gibson, J. A., Rich, J., et al. (2013) High level of intergenera gene exchange shapes the evolution of haloarchaea in an isolated Antarctic lake. Proc Natl Acad Sci USA 110: 16939-16944. Tschitschko, B., Williams, T. J., Allen, M. A., Páez-Espino, D., Kyrpides, N., Zhong, L., et al. (2015) Antarctic archaea–virus interactions: metaproteome-led analysis of invasion, evasion and adaptation. ISME J 9: 2094-2107. Tschitschko, B., Williams, T. J., Allen, M. A., Zhong, L., Raftery, M. J., and Cavicchioli, R. (2016) Ecophysiological distinctions of haloarchaea from a hypersaline Antarctic lake determined using metaproteomics. Appl Environ Microbiol 82: 3165 – 3173. Williams, T. J., Allen, M. A., DeMaere, M. Z., Kyrpides, N. C., Tringe, S. G., Woyke, T., and Cavicchioli, R. (2014) Microbial ecology of an Antarctic hypersaline lake: genomic assessment of ecophysiology among dominant haloarchaea. ISME J 8: 1645-1658.

2017-02-27 | PXD005076 | Pride

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Project description:The investigators recently identified promiscuous HLA-DR-derived epitopes from the human telomerase reverse transcriptase (TERT) called universal cancer peptides (UCP), to study tumor-specific CD4+ T cell responses. The investigators found high frequency of naturally occuring UCP-specific TH1 cells in long term survival of metastatic colorectal cancer (CRC) previously treated by 5 fluoro-uracil -oxaliplatin (Folfox) +/- bevacizumab regiment (Godet et al. OncoImmunology 2012 and unpublished data). Epitopes-CRC02 is a French prospective multicenter study which will evaluate the post chemotherapy and post surgery modulation of host tumor-specific CD4 TH1 cell responses in metastatic colorectal cancer patients and their correlation with progression-free survival.

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