Project description:Glutamicibacter arilaitensis grown in large scale liquid Cheese Curd media and extracted with Amberlite XAD16, back extracted with MeOH:DCM 50:50, separated on SPE column to afford 7 fractions labeled A through G based on increasing polarity (A = 10% MeOH, F = 100% MeOH and G = 100% EtoAC. Fractions E(60% MeOH), D(80% MeOH), and F(100% MeOH) run on a qTOF and submitted here. suffix 1 refers to treatment of cultures with desferrioxamine and suffix PLAIN means untreated. JB182new_20170614 is a crude extract from a subsequent small scale growup without desferrioxamine.
Project description:Although a seemingly harmless developmental stage of herbivores, insect eggs trigger efficient plant defenses that include necrosis, callus formation, accumulation of ovicidal compounds and release of volatiles to attract egg predators. The large white butterfly Pieris brassicae deposits batches of 20-30 eggs onto Arabidopsis leaves, causing a large transcriptional reprograming that is drastically distinct from the expression profile triggered by larval feeding. Also, P. brassicae eggs induce localized cell death, accumulation of reactive oxygen species (ROS) and salicylic acid (SA), and expression of PTI-related genes, suggesting that egg-associated molecular patterns (EAMPs) activate a response that is similar to the response induced by microbial pathogens. We previously reported that a crude P. brassicae egg extract (EE, soluble fraction from crushed eggs) induced similar responses as oviposition, including ROS and SA accumulation, cell death and defense gene induction. In order to compare oviposition and EE treatment at the transcriptome level, we analyzed changes in transcipt abundance with P. brassicae EE or after natural oviposition. After 5 days, hundreds of genes were significantly upregulated by each treatment and their induction was highly similar between treatments. This conserved transcriptomic signature thus strongly supports our previous observations that oviposition and EE treatment trigger comparable responses in Arabidopsis.
Project description:Enzymatic Methyl-seq (EM-seq) is an enzyme-based method giving us reliable methylome data from small amounts of purified DNA. However, it is unclear whether EM-seq library can be constructed from crude cell lysate containing genomic DNA. We evaluated the quality of EM-seq libraries directly prepared from crude cell lysate.
Project description:We investigated the effects of the crude extract of a South African medicinal plant, Cotyledon orbiculata, on cell survival of colon (HCT116) cancer cell lines. Using RNASeq, we discovered that the extract interfered with mRNA regulatory pathways. Here, we found that the extract of Cotyledon orbiculata, a South African medicinal plant, had an anti-proliferative effect in cancer cells, mediated by apoptosis induced by alternative splicing of hnRNPA2B1 and BCL2L1.
Project description:In order to get mechanistic insights into the cardiomyogenic and differentiation-promoting activity of Crataegus spp. Extract WS®1442 and the identified bioactive subfractions (MeOH eluate), we have employed whole genome microarray expression profiling after 6 hours and 24 hours of treatment. We found that stress-associated genes are affected as well as specific signaling pathways such as TGFbeta, FGF, BDNF and retinoic acid.