Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

GNPS - Fe-Nanoparticle for enrichment of Siderophore; washes, eluate, sup

ABSTRACT: Iron nanoparticles are effective in enriching siderophore from complex microbiome

INSTRUMENT(S): QExactive LCMS

ORGANISM(S): Pseudomonas

SUBMITTER: Allegra Aron

PROVIDER: MSV000096305 | GNPS | Tue Nov 05 20:58:00 GMT 2024

REPOSITORIES: GNPS

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
		Other

Items per page:

1 - 1 of 1

Similar Datasets

Fe-Nanoparticle for enrichment of Siderophore; washes, eluate, sup

Project description:Iron nanoparticles are effective in enriching siderophore from complex microbiome

2024-11-05 | MSV000096305 | MassIVE

Fe-Nanoparticle for enrichment of Siderophore

Project description:Fe Nanoparticle is an effective technique for enriching siderophores from complex microbiome

2024-10-31 | MSV000096267 | MassIVE

GNPS - Fe-Nanoparticle for enrichment of Siderophore

Project description:Fe Nanoparticle is an effective technique for enriching siderophores from complex microbiome

2024-10-31 | MSV000096267 | GNPS

The Serratia marcescens siderophore, serratiochelin, is necessary for full virulence during bloodstream infection

Project description:Serratia marcescens is a bacterium frequently found in the environment, but over the last several decades it has evolved into a concerning clinical pathogen, causing fatal bacteremia. To establish such infections, pathogens require specific nutrients; one very limited but essential nutrient is iron. We sought to characterize the iron acquisition systems in S. marcescens isolate UMH9, which was recovered from a clinical bloodstream infection. Using RNA sequencing (RNA-seq), we identified two predicted siderophore gene clusters (cbs and sch) that were regulated by iron. Mutants were constructed to delete each iron acquisition locus individually and in conjunction, generating both single and double mutants for the putative siderophore systems. Mutants lacking the sch gene cluster lost their iron-chelating ability as quantified by the chrome azurol S (CAS) assay, whereas the cbs mutant retained wild-type activity. Mass spectrometry-based analysis identified the chelating siderophore to be serratiochelin, a siderophore previously identified in Serratia plymuthica. Serratiochelin-producing mutants also displayed a decreased growth rate under iron-limited conditions created by dipyridyl added to LB medium. Additionally, mutants lacking serratiochelin were significantly outcompeted during cochallenge with wild-type UMH9 in the kidneys and spleen after inoculation via the tail vein in a bacteremia mouse model. This result was further confirmed by an independent challenge, suggesting that serratiochelin is required for full S. marcescens pathogenesis in the bloodstream. Nine other clinical isolates have at least 90% protein identity to the UMH9 serratiochelin system; therefore, our results are broadly applicable to emerging clinical isolates of S. marcescens causing bacteremia.

2020-05-24 | GSE145587 | GEO

TiO2 Nanoparticle for Siderophore Extraction

Project description:Novel and more efficient technique for selectively enriching siderophore from complex microbiome

2024-07-30 | MSV000095484 | MassIVE

Fe-Nanoparticle for enrichment of Siderophore

Project description:Fe Nanoparticle serves as an effective technique for the enrichment of siderophores from pseudomonas

2024-10-31 | MSV000096266 | MassIVE

GNPS - Fe-Nanoparticle for enrichment of Siderophore

Project description:Fe Nanoparticle serves as an effective technique for the enrichment of siderophores from pseudomonas

2024-10-31 | MSV000096266 | GNPS

The proteomic response of a marine Vibrio to low iron and desferrioxamine siderophore bioavailability

Project description:Competition for limited iron resources is a key driver of microbial community structure in many regions of the surface ocean. The bacterial siderophores ferrioxamine and amphibactin have been identified in marine surface waters, suggesting that they may represent an important bacterial strategy for obtaining iron from a scarcely populated pool. We screened several strains of marine Vibrio for the presence of putative amphibactin biosynthesis gene homologues and amphibactin production. Whole cell proteomics, siderophore isolation, and isotopically labeled iron uptake experiments were performed. Here, we show that an amphibactin-producing marine bacterium, Vibrio cyclitrophicus str. 1F-53, harbors an independently regulated uptake pathway for ferrioxamines. Proteomic analyses identified upregulation of the amphibactin NRPS system and a putative amphibactin siderophore transporter in response to low iron concentrations. In addition, multiple other transporters were upregulated, however when desferrioxamine was present, amphibactin production decreased and the ferrioxamine receptor increased in abundance. Such cheating phenotypes, which appear widespread among marine amphibactin producers, highlight the strategies that contribute to the fitness of marine bacteria in the face of iron stress. These results demonstrate siderophore producer and cheater phenotypes and highlight the cellular restructuring which is involved due to competition for iron, that shapes the community structure of marine ecosystems.

2021-04-19 | PXD011427 | Pride

GNPS - TiO2 Nanoparticle for Siderophore Extraction

Project description:Novel and more efficient technique for selectively enriching siderophore from complex microbiome

2024-07-30 | MSV000095484 | GNPS

A role for PchHI as an ABC transporter in iron acquisition by the siderophore pyochelin in Pseudomonas aeruginosa

Project description:Iron is an essential nutrient for bacterial growth but poorly bioavailable. To scavenge ferric iron present in their environment, bacteria synthesize and secrete siderophores, small compounds with a high affinity for iron. Pyochelin (PCH) is one of the two siderophores produced by the opportunistic pathogen Pseudomonas aeruginosa. Once having captured a ferric iron, PCH-Fe is imported back into bacteria first by the outer membrane transporter FptA and afterwards by the inner membrane permease FptX. Here using molecular biology, 55Fe uptake assays and LC-MS/MS quantification of PCH in the different bacterial cell fractions, we show that (i) PCH (probably under its PCH-Fe form) is able to rich bacterial periplasm and cytoplasm when both FptA and FptX are expressed, and (ii) that PchHI (a heterodimeric ABC transporter) plays a role in the translocation of siderophore-free iron siderophore-free iron across the inner membrane into the cytoplasm. Consequently, probably the first fraction of PCH-Fe internalized by FptA may be transported further by FptX in the bacterial cytoplasm to activate the transcriptional regulator PchR, regulating the transcription of all genes of the PCH pathway. The further fractions of PCH-Fe transported by FptA may dissociate in the bacterial periplasm by an unknown mechanism, with the siderophore-free iron being transported into the cytoplasm by PchHI.

2022-03-15 | PXD026833 | Pride

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data