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The role of ptpn21 in hematopoietic stem cells

ABSTRACT: To identify the downstream substrate/effector that mediates Ptpn21 function. We applied comprehensive phosphoproteomics analysis with Lin- cells isolated from Ptpn21-/- and Ptpn21+/+ mice. The analysis revealed many pTyr sites on molecules associated with cytoskeleton. Additionally, we present a list of Ptpn21-dependent targets and putative substrates including Septin1 with increased tyrosine phosphorylation.

ORGANISM(S): Mus Musculus

SUBMITTER: Chengkui Qu

PROVIDER: PXD011381 | iProX | Wed Oct 17 00:00:00 BST 2018

REPOSITORIES: iProX

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Ptpn21 Controls Hematopoietic Stem Cell Homeostasis and Biomechanics.

Ni Fang F Yu Wen-Mei WM Wang Xinyi X Fay Meredith E ME Young Katherine M KM Qiu Yongzhi Y Lam Wilbur A WA Sulchek Todd A TA Cheng Tao T Scadden David T DT Qu Cheng-Kui CK

Cell stem cell 20190314 4

Hematopoietic stem cell (HSC) quiescence is a tightly regulated process crucial for hematopoietic regeneration, which requires a healthy and supportive microenvironmental niche within the bone marrow (BM). Here, we show that deletion of Ptpn21, a protein tyrosine phosphatase highly expressed in HSCs, induces stem cell egress from the niche due to impaired retention within the BM. Ptpn21<sup>-/-</sup> HSCs exhibit enhanced mobility, decreased quiescence, increased apoptosis, and defective reconst ...[more]

PMID: 30880025

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