Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

2 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Mechanism of sterility and fertility restoration in C-type cytoplasm male sterility maize by different omics methods

ABSTRACT: detection of gene changes in anther development among sterile, maintainer and restorer lines

ORGANISM(S): Zea Mays

SUBMITTER: Yadong Xue

PROVIDER: PXD016033 | iProX | Mon Oct 28 00:00:00 GMT 2019

REPOSITORIES: iProX

ACCESS DATA

Json Xml

Publications

Comparative proteomic analysis of mitochondrial proteins from maize CMS-C sterile, maintainer and restorer anthers.

Zhang Huaisheng H Wang Bin B Li Bing B Lin Yanan Y Yang Huili H Ding Dong D Xue Yadong Y Tang Jihua J

The plant genome 20200522 2

The maize C system of cytoplasmic male sterility (CMS) and its fertility restoration gene Rf4 have been widely used for maize hybrid production; however, the underlying mechanism is still uncertain. The sterility factor functions in mitochondria, where it interacts directly or indirectly with the restorer. Mitoproteomics can capture all participants involved in CMS and restoration at the organelle level. In the present study, we identified and quantified anther mitochondrial proteins from CMS, m ...[more]

PMID: 33016607

Similar Datasets

Spatiotemporal and cell-type dependent phasiRNA biogenesis in maize male reproduction (PARE)

Project description:Maize anthers, the male reproductive floral organs, express two classes of phased, small interfering RNAs (phasiRNAs). RNA profiling from ten sequential cohorts of staged maize anthers plus mature pollen revealed that 21-nt phased siRNAs (21-phasiRNAs) from 463 loci appear abruptly after germinal and initial somatic cell fate specification and then diminish, while 24-nt phased siRNAs (24-phasiRNAs) from 176 loci coordinately accumulate during meiosis and persist as haploid gametophytes differentiate into pollen. RNA sequencing of anther developmental mutants, together with in situ RNA hybridization detection of phasiRNA biogenesis factors, demonstrated that 21-phasiRNAs and 24-phasiRNAs are independently regulated. Furthermore, 21-phasiRNAs require epidermal cells while 24-phasiRNAs require functional tapetal cells. Maize phasiRNAs and mammalian PIWI-interacting RNAs (piRNAs) illustrate convergent evolution of small RNAs to support male reproduction.

2015-02-11 | GSE52297 | GEO

Optimization of protein extraction methods for root-associated microbes in maize

Project description:The goal of this study was to optimize protein extraction methods to study root-associated bacteria in maize. For this we inoculated sterile maize plants with a synthetic community composed of seven different bacteria (Ben Niu et al. PNAS 2017, vol 114, n 12). Then, we extracted proteins from maize roots using eight different protein extraction methods in triplicates. These methods were a combination of different extraction buffers (SDS or Triton-based) and mechanical disruption methods (bead-beating, N2 grinding, glass homogenizer and freeze-thaw cycles). We found that vortexing maize roots with glass beads in PBS yielded the highest numbers of microbial protein identification.

2022-09-08 | PXD026369 | Pride

Spatiotemporal and cell-type dependent phasiRNA biogenesis in maize male reproduction (RNA-seq)

2015-02-11 | GSE52290 | GEO

Spatiotemporal and cell-type dependent phasiRNA biogenesis in maize male reproduction (RNA-seq2)

2020-05-15 | GSE150597 | GEO

Spatiotemporal and cell-type dependent phasiRNA biogenesis in maize male reproduction (small RNA-seq)

2015-02-11 | GSE52293 | GEO

Spatiotemporal and cell-type dependent phasiRNA biogenesis in maize male reproduction (PARE)

2015-02-11 | E-GEOD-52297 | biostudies-arrayexpress

label-free analysis of male-sterile line 1355A of cotton

Project description:Cotton (Gossypium spp.) is one of the most important economic crops and exhibits yield-improving heterosis in specific hybrid combinations. The genic male-sterility system is the main strategy used for producing heterosis in cotton. To better understand the mechanisms of male sterility in cotton, we carried out label-free quantitative proteomics analysis in the anthers of two near-isogenic lines, the male-sterile line 1355A and the male-fertile line 1355B, the two isogenic lines. These experiments, along with bioinformatics analyses, identified 124 proteins from the anthers at uninucleate pollen stage (stage 8) that were significantly differentially expressed between these two lines.

2019-03-19 | PXD012398 | Pride

Spatiotemporal and cell-type dependent phasiRNA biogenesis in maize male reproduction (RNA-seq)

2015-02-11 | E-GEOD-52290 | biostudies-arrayexpress

Proteomic Changes of Wheat Male Sterile Anther Induced by Temperature

Project description:Wheat, an important human food crop, is very sensitive to temperature changes. Thermosensitive sterile lines (TMS) is a natural material for exploring the effects of anther development and temperature changes on male fertility. In order to study the possible molecular mechanism of regulating protein activity during anther fertility alteration, proteomics analysis based on high resolution mass spectrometry were used to study the binuclear anther of wheat YS3038.

2022-08-12 | PXD029487 | Pride

Spatiotemporal and cell-type dependent phasiRNA biogenesis in maize male reproduction (small RNA-seq)

2015-02-11 | E-GEOD-52293 | biostudies-arrayexpress

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data