Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

1 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Comparative Proteomics Indicates Metformin Ameliorates Endometrial Receptivity of infertile women with Endometriosis

ABSTRACT: Background: Endometriosis affects approximately 10% of reproductive age women and 30%-50% of infertile women. Decreased endometrial receptivity is mainly responsible for infertile women with minimal/mild endometriosis. Medical therapy to improve fecundity of these women is needed. Metformin is reported to inhibit growth of ectopic lesions in endometriosis, while the effect on eutopic endometrium is unclear. The study was to identify if metformin had effect on eutopic endometrium in women with minimal/mild endometriosis and thus improve their fecundity. Materials and Methods: Paired endometrial tissues of secretory phase from participants with minimal/mild endometriosis were collected before and after two months of metformin treatment and setting metformin-free control. Protein expression profiles of endometrium were analyzed by proteomics. Results: A total of 115 proteins were detected up-regulated after metformin therapy, including Insulin-like growth factor-binding protein 7, α-antitrypsin, apolipoprotein D, Rho GDP-dissociation inhibitor 1, brain form glycogen phosphorylase, and Cathepsin B that associated with endometrial receptivity, while there had no significant changes in controls. These up-regulated markers were validated through target proteomics and further demonstrated with endometriosis mice models. Conclusions: This study revealed that metformin might ameliorate biomarkers expressions of endometrial receptivity in human and mice model. Metformin is potential to improve endometrial receptivity of endometriosis-associated infertility.

ORGANISM(S): Homo Sapiens

SUBMITTER: Wei Huang

PROVIDER: PXD027648 | iProX | Thu Jul 29 00:00:00 BST 2021

REPOSITORIES: iProX

ACCESS DATA

Json Xml

Similar Datasets

Single-cell Transcriptomics of Endometrial Cells from Patients with Minimal/mild Endometriosis.

Project description:We profiled the transcriptomes of 128, 243 endometrial cells from 6 infertile minimal/mild endometriosis patients and 4 controls. Our results provide new insight of pathogenesis and impaired endometrial receptivity of endometriosis.

2023-03-28 | GSE214411 | GEO

Loss of endometrial plasticity in recurrent pregnancy loss (RNA-Seq)

Project description:In order to try and identify characteristics of gene expression in the endometrium of women suffering infertility or recurrenty miscarriage, we performed RNAseq on endometrial pipelle biopsies from 20 women. The endometrial transcriptome in the mid-luteal phase of the cycle (window of implantation) is highly divergent in women suffering infertility or miscarriages. 20 mid-luteal endometrial biopsies were analysed from infertile women and patients suffering recurrent pregnancy loss.

2015-09-16 | E-GEOD-65099 | biostudies-arrayexpress

Natural cycle frozen embryo transfer is superior to artificial cycle in women with recurrent implantation failure: endometrial transcriptome study

Project description:Transcriptome profile of receptive phase endometrium among infertile women with recurrent implantation failure (RIF) in two different endometrial preparation protocols for FET was analyzed: natural cycle (NC-FET) vs. artificial cycle (AC-FET). Fifteen endometrial biopsy samples were obtained: women with unexplained RIF (n = 5) in natural cycles for FET (NC-FET), women with unexplained RIF undergoing artificial endometrial preparation (n = 5) for FET (AC-FET), and healthy women (n = 5) with proven fertility in natural cycles (NC-FC) (Control group). All endometrial biopsies were obtained during the mid-secretory phase, at the time of ‘window of implantation’.

2016-01-15 | E-MTAB-3655 | biostudies-arrayexpress

Natural cycle frozen embryo transfer is superior to artificial cycle in women with recurrent implantation failure: endometrial transcriptome study

2015-10-28 | E-MTAB-3713 | biostudies-arrayexpress

Poor Endometrial Proliferation after Clomiphene is Associated with Altered Estrogen Action [RNA-Seq]

Project description:We report transcriptomic profiles from endometrial biopsies obtained from infertile women in the late proliferative phase who developed either optimal (≥8mm) or suboptimal (<6mm) endometrial thickness following early follicular phase exposure to 100mg clomiphene citrate for 5 days.

2021-06-15 | GSE164768 | GEO

Poor Endometrial Proliferation after Clomiphene is Associated with Altered Estrogen Action [ChIP-Seq]

Project description:We report ESR1 cistromic profiles from endometrial biopsies obtained from infertile women in the late proliferative phase who developed either optimal (≥8mm) or suboptimal (<6mm) endometrial thickness following early follicular phase exposure to 100mg clomiphene citrate for 5 days.

2021-06-15 | GSE164764 | GEO

Endometrial gene expression differences in women with coronavirus disease 2019

Project description:Objective: To study the potential effect of COVID-19 on the endometrium of affected symptomatic women. Design: Preliminary study of the endometrial transcriptomes in women with COVID-19 through RNA sequencing. Setting: Hospital and university laboratories. Subjects: Women with COVID-19 lacking SARS-CoV-2 infection in endometrial tissue. Intervention/Exposure: Endometrial biopsy collection. Main outcomes measures: Endometrial gene expression and functional analysis of patients with COVID-19 versus uninfected individuals. Results: COVID-19 systemic disease alters endometrial gene expression in 75% of women, with patients exhibiting a preponderance of 163 up-regulated (e.g., UTS2, IFI6, IFIH1, BNIP3) and 72 down-regulated genes (e.g., CPZ, CDH3, IRF4) (FDR<0.05). A total of 161 dysregulated functions (36 up-regulated and 125 down-regulated) were typically enriched in COVID-19 endometria, including upregulation in pathways involved in response to virus and cytokine inflammation, highlighting upregulation of a COVID-19 response pathway. Conclusion: COVID-19 affects endometrial gene expression despite the absence of SARS-CoV-2 particles in endometrial tissues.

2024-04-22 | GSE202553 | GEO

Endometrial microbiota in infertile women with and without chronic endometritis

Project description:Endometrial microbiota in infertile women with and without chronic endometritis

| PRJNA530321 | ENA

Investigation of placental gene expression in obese women exposed to Metformin during pregnancy

Project description:Maternal obesity in pregnancy is associated with increased birth-weight, obesity and premature mortality in adult offspring. The Effect of Metformin on Maternal and Fetal Outcomes in Pregnant Obese Women (EMPOWaR) trial was a randomised, double-blind, placebo-controlled trial carried out to determine whether exposure to Metformin would affect the offspring birth-weight centile. Obese women exposed to Metformin had increased insulin sensitivity at 36 weeks of pregnancy, but there were no differences in offspring birthweight. We obtained the placentas from these women to determine whether there were differences in expression of genes regulating fetal growth and metabolism. In a complementary study we investigated DNA methylation in the same samples.

2019-01-31 | E-MTAB-6418 | biostudies-arrayexpress

Investigation of placental DNA methylation in obese women exposed to Metformin during pregnancy

Project description:Maternal obesity in pregnancy is associated with increased birth-weight, obesity and premature mortality in adult offspring. The Effect of Metformin on Maternal and Fetal Outcomes in Pregnant Obese Women (EMPOWaR) trial was a randomised, double-blind, placebo-controlled trial carried out to determine whether exposure to Metformin would affect the offspring birth-weight centile. Obese women exposed to Metformin had increased insulin sensitivity at 36 weeks of pregnancy, but there were no differences in offspring birthweight. We obtained the placentas from these women to determine whether there were differences in DNA methylation of genes regulating fetal growth and metabolism. In a related study we investigated the gene expression in the same samples.

2018-11-01 | E-MTAB-6421 | biostudies-arrayexpress

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data