Ontology highlight
ABSTRACT:
ORGANISM(S): Homo Sapiens
SUBMITTER: Chen Ding
PROVIDER: PXD028104 | iProX | Wed Aug 25 00:00:00 BST 2021
REPOSITORIES: iProX
Analytical chemistry 20161130 24
The ability to map endogenous transcription factors (TFs) DNA binding activity at the proteome scale will greatly enhance our understanding of various biological processes. Here we report a highly sensitive, rapid, and high-throughput approach, transcription factor response elements on tip-mass spectrometry (TOT-MS), that allows for quantitative measurement of endogenous TFs. A total of 150 TFs from 1 μg of nuclear extracts can be quantified with single shot mass spectrometry detection in 1 h of ...[more]