Dataset Information

Proteome response to drought stress in different sweetpotato cultivars

ABSTRACT: A greenhouse pot experiment was conducted at experimental station. Drought treatment was conducted after transplanting for 10 days. seedlings were subjected to stress for about 20 days withholding irrigation until SMC was reduced to around 8%. The third full expanded leaves from the top were used for proteomic experiment. Fresh leaves were flash frozen in liquid nitrogen separately and stored at -80 °C for protein extraction.

ORGANISM(S): Dioscorea Esculenta

SUBMITTER: Daifu Ma

PROVIDER: PXD028232 | iProX | Wed Sep 08 00:00:00 BST 2021

REPOSITORIES: iProX

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Publications

Differential response of physiology and metabolic response to drought stress in different sweetpotato cultivars.

Zhou Zhilin Z Tang Jun J Cao Qinghe Q Li Zongyun Z Ma Daifu D

PloS one 20220310 3

Sweetpotato (Ipomoea batatas [L.] Lam) is a widely cultivated food crop with generally good adaptability. However, drought stress can cause a significant decline in yield. To reveal the response mechanism of sweetpotato to drought stress, an integrated physiological, proteomic and metabolomic investigation was conducted in leaves of two sweetpotato varieties with differing responses to drought stress, drought-resistant Wanzishu56 (WZ56) and a more sensitive variety, Ningzishu2(NZ2). Physiologica ...[more]

PMID: 35271628

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Project description:The experiment was carried out during two vegetation seasons from 25th of April to 10th of September. Two Polish potato cultivars, the drought-tolerant Gwiazda and drought-sensitive Oberon were used. Selected tubers with transverse diameters of 3 - 4 cm were pre-sprouted for 2 weeks before planting. Plants were grown in a vegetation hall in pots filled with a thin layer of gravel on the bottom and the universal vegetable soil substrate ‘Hollas’ (Agaris Polska Ltd., Poland) produced from peat with the addition of chalk at a pH range of 5.5-6.5. Additionally, in phase 20 of the BBCH-scale of plant development, MIS-3 (Intermag) fertilizer was applied. Water content (WC) in volumetric basis in soil pots was measured according Black (1965). Weather conditions during the years of study were monitored using a Weather Campbell Station (Campbell Scientific Inc.) located in close proximity, and a thermohygrograph placed between pots. Meteorological data of air temperature, the photosynthetically active radiation, and humidity were comparable in the years of study and favorable for potato development. Three weeks after the initiation of the tuberisation phase (56 DAP), plants were divided into 3 groups, each consisting of 6 plants. The first group of plants was subjected to soil drought (remained without irrigation, day/night temperature 22°C/18°C), the second one to high temperature (day/night temperature 38°C/25°C) and the third one was watered according to needs and at optimal temperature (control plants, day/night temperature 22°C/18°C). Stress application lasted 14 days and finished at 70 DAP. During this period, plants were placed in phytotron equipped with six Hortilux Schreder Lamps with Philips light bulbs of 1600 W each. Air humidity was in the range 65-70%. WC under 14 days of soil drought reached 30% (v/v), and remained 80% (v/v) in control and high temperature conditions. During the recovery period, after 14 days of stress treatment, WC reached control levels. Plant root material for proteomic research was collected on the 14th day of stress treatment (70 DAP).

Dataset Information

Proteome response to drought stress in different sweetpotato cultivars

Publications

Differential response of physiology and metabolic response to drought stress in different sweetpotato cultivars.

Similar Datasets

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