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Selective enrichment of N-terminal proline peptides via hydrazide chemistry for proteomics analysis

ABSTRACT: A challenge for shotgun proteomics is the identification of low abundance proteins, which is always hampered owing to the extreme complexity of protein digests and highly dynamic concentration range of proteins. To reduce the complexity of the peptide mixture, we developed a novel method to selectively enrich N-terminal proline peptides via hydrazide chemistry. This method consisted of ortho-phthalaldehyde (OPA) blocking of primary amines in peptides, reductive glutaraldehydation of N-terminal proline and solid phase hydrazide chemistry enrichment of aldehyde-modified N-terminal proline peptide.

ORGANISM(S): Homo Sapiens

SUBMITTER: Weibing Zhang

PROVIDER: PXD029178 | iProX | Mon Oct 18 00:00:00 BST 2021

REPOSITORIES: iProX

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Selective enrichment of N-terminal proline peptides via hydrazide chemistry for proteomics analysis.

Li Zhouxian Z Wang Qi Q Mao Jiawei J Zhang Lingyi L Zhang Weibing W Ye Mingliang M

Analytica chimica acta 20201023

A challenge for shotgun proteomics is the identification of low abundance proteins, which is always hampered owing to the extreme complexity of protein digests and highly dynamic concentration range of proteins. To reduce the complexity of the peptide mixture, we developed a novel method to selectively enrich N-terminal proline peptides via hydrazide chemistry. This method consisted of ortho-phthalaldehyde (OPA) blocking of primary amines in peptides, reductive glutaraldehydation of N-terminal p ...[more]

PMID: 33280703

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