Dataset Information

Algicidal interaction between the soil bacterial isolate Paenibacillus polymyxa MEZ6

ABSTRACT: We performed a label-free proteomics studies to reveal the responses of MEZ6 to the presence of 21gr at protein expression level. The total proteins of MEZ6 were extracted 1 day after co-inoculation and the proteomics samples were prepared.

ORGANISM(S): Paenibacillus Polymyxa

SUBMITTER: Bo Xie

PROVIDER: PXD029555 | iProX | Thu Nov 04 00:00:00 GMT 2021

REPOSITORIES: iProX

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Publications

Algicidal interaction between Paenibacillus polymyxa MEZ6 and microalgae.

Zhao Na N Yi Li L Ren Sanguo S Yin Qin Q Xiang Wei W Zhang Xu X Xie Bo B

Journal of applied microbiology 20220504 2

<h4>Aims</h4>Algicidal bacteria can be used for control of harmful algal bloom and extraction of algal bioproducts based on their algae-lysing activities. This work investigated the algae-lysing activity of a newly isolated algicidal bacterium, Paenibacillus polymyxa strain MEZ6 and its possible mechanisms.<h4>Methods and results</h4>Algicidal bacteria were isolated from soil samples collected at the university campus. Co-inoculation tests identified that one isolate, MEZ6, can rapidly kill euka ...[more]

PMID: 35462459

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Project description:Inducible co-stimulator (ICOS) interaction with its ligand (ICOSL) is involved in several T cell effector functions. While blockade of ICOS:ICOSL interaction in chronic graft versus host disease (GVHD) seems benefi cial, results for acute GVHD remain controversial. To further elucidate its role in acute GVHD, C57BL / 6 mice were lethally irradiated and reconstituted with allogeneic spleen cells in the absence or presence of ICOSL-blocking mAb. Mice reconstituted with allogeneic spleen cells experienced severe GVHD and died untreated within 6 M-bM-^@M-^S 9 days after transplantation. Mice treated with an anti-ICOSL mAb starting from day 3 after transplantation gained weight again and survived for at least additional 12 days, although the treatment was already stopped at day 11 after transplantation. In contrast, the anti-ICOSL treatment starting from day 0 did not prevent GVHD. The diff erence between therapeutic (day 3) and prophylactic (day 0) anti-ICOSL treatment was independent of CD25 + CD4 + regulatory T cells since their depletion did not abrogate the therapeutic eff ect of ICOSL blockade. Microarray analysis revealed IFN- M-NM-3 and chemokine up-regulation in spleen cells of prophylactically treated mice, emphasizing kinetic dependence of acute GVHD modulation via blockade of ICOS:ICOSL interaction. B6 recipients were irradiated with 10.0 Gray, administered from a 137 Cs source. Splenocytes from C3H mice were prepared as single cell suspensions in PBS, depleted of red blood cells and counted. 2 M-bM-^@M-^S 3 M-CM-^W 10^7 C3H splenocytes in a volume of 200 M-NM-<l were transplanted into B6 recipients via tail vein injection (4 mice per group per experiment) 4 M-bM-^@M-^S 6 h after irradiation. Mice in the treatment group with anti-ICOSL mAb and their respective controls, received 500 M-NM-<g mAb i. p starting at day 0 or day 3, followed by subsequent injections of 200 M-NM-<g of mAb every other day. At day 4 after transplantation RNA of spleen cells was prepared and subjected to microarray analysis. Combined RNA from allogeneic transplanted mice was hybridized onto 2 independent arrays.

Dataset Information

Algicidal interaction between the soil bacterial isolate Paenibacillus polymyxa MEZ6

Publications

Algicidal interaction between Paenibacillus polymyxa MEZ6 and microalgae.

Similar Datasets

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