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High-throughput lysis and proteolytic digestion of tissues using pressure cycling technology for proteomics

ABSTRACT: we describe a protocol for pressure cycling technology (PCT) assisted tissue sample preparation for high-throughput proteomics

ORGANISM(S): Homo Sapiens Mus Musculus

SUBMITTER: Tiannan Guo

PROVIDER: PXD030645 | iProX | Fri Dec 24 00:00:00 GMT 2021

REPOSITORIES: iProX

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High-throughput proteomic sample preparation using pressure cycling technology.

Cai Xue X Xue Zhangzhi Z Wu Chunlong C Sun Rui R Qian Liujia L Yue Liang L Ge Weigang W Yi Xiao X Liu Wei W Chen Chen C Gao Huanhuan H Yu Jing J Xu Luang L Zhu Yi Y Guo Tiannan T

Nature protocols 20220805 10

High-throughput lysis and proteolytic digestion of biopsy-level tissue specimens is a major bottleneck for clinical proteomics. Here we describe a detailed protocol of pressure cycling technology (PCT)-assisted sample preparation for proteomic analysis of biopsy tissues. A piece of fresh frozen or formalin-fixed paraffin-embedded tissue weighing ~0.1-2 mg is placed in a 150 μL pressure-resistant tube called a PCT-MicroTube with proper lysis buffer. After closing with a PCT-MicroPestle, a batch o ...[more]

PMID: 35931778

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