Dataset Information

Proteomics data of muscle tissue from triploid crucian crap, red crucian crap, common crap and allotetraploid

ABSTRACT: Quantitative of muscle tissue proteomics profiling in triploid fish, red crucian carp, common carp and allotetraploid were obtained by SWATH- MS

ORGANISM(S): Allotetraploid Cyprinus Carpio X Carassius Auratus Red Var.

SUBMITTER: Shaojun Liu

PROVIDER: PXD030692 | iProX | Sat Jan 01 00:00:00 GMT 2022

REPOSITORIES: iProX

ACCESS DATA

Similar Datasets

Project description:Training at sustainable swimming speeds can produce changes in fish skeletal muscle that are important for aquaculture due to their growth-potentiating effects. Such changes may be even more relevant when fish are fed diets containing an increasing proportion of carbohydrates as an energy source. We evaluated the effects of moderate-intensity sustained swimming on the transcriptomic response of red and white muscle in rainbow trout fed a carbohydrate-rich diet using microarray and qPCR. Analysis of the red and white muscle transcriptome revealed significant changes in the expression of a large number of genes (395 and 597, respectively), with a total of 218 differentially expressed genes (DEGs) common for both muscles. A large number of the genes involved in glucose use and energy generation, contraction, development, synthesis and catabolism of proteins were up-regulated in red and white muscle. Additionally, DEGs in both muscles were involved in processes of defense response and apoptosis. Skeletal muscle contraction activates a transcriptional program required for the successful adaptation of both muscles to the changing demands imposed by swimming conditions. Future studies should further clarify the mechanisms involved in the adaptation of both tissues to exercise and assess possible benefits of such conditions for cultured fish. Total RNA from pooled red and white skeletal muscle samples of individual rainbow trout from each group (resting fish, n=8; swimming fish, n=8) was labeled with Cy3-dUTP and Cy5-dUTP (GE Healthcare, Barcelona, Spain). We used a dye swap experimental design and each cDNA from a pooled RNA sample was hybridized to two microarrays. For the first slide, cDNAs from resting and swimming fish were labeled with Cy5 and Cy3, respectively, and for the second array dye assignment was reversed. Therefore, samples from individual fish within each group were pooled and expression values shown represent the means of 6 M-CM-^W 2 = 12 technical replicates. A total of four slides were used in this study

Dataset Information

Proteomics data of muscle tissue from triploid crucian crap, red crucian crap, common crap and allotetraploid

Similar Datasets

OmicsDI is part of the ELIXIR infrastructure