Ontology highlight
ABSTRACT:
ORGANISM(S): Homo Sapiens
SUBMITTER: Ruijun Tian
PROVIDER: PXD039316 | iProX | Sat Oct 07 00:00:00 BST 2023
REPOSITORIES: iProX
Weng Yicheng Y Chen Wendong W Kong Qian Q Wang Ruixiang R Zeng Ruxin R He An A Liu Yanjun Y Mao Yiheng Y Qin Yunqiu Y Ngai William Shu Ching WSC Zhang Heng H Ke Mi M Wang Jie J Tian Ruijun R Chen Peng R PR
Nature chemical biology 20240102 5
Cellular context is crucial for understanding the complex and dynamic kinase functions in health and disease. Systematic dissection of kinase-mediated cellular processes requires rapid and precise stimulation ('pulse') of a kinase of interest, as well as global and in-depth characterization ('chase') of the perturbed proteome under living conditions. Here we developed an optogenetic 'pulse-chase' strategy, termed decaging kinase coupled proteomics (DeKinomics), for proteome-wide profiling of kin ...[more]