Dataset Information

Proteome alterations in the lung of Beagle dogs during the lung migration stage of Toxocara canis infection

ABSTRACT: Background: Toxocara canis is a prevalent helminth, which threatens millions of people worldwide with a predilection for pediatric and adolescent populations in impoverished communities. Exploring the invasion and developmental mechanisms associated with T. canis infection in its definitive hosts will help to better control and eliminate the effects of toxocariasis. Methods: To investigate the molecular mechanism of Beagle dogs' response to T. canis infection, proteomic alterations in the lung of Beagle puppies were systematically analyzed by data-independent acquisition (DIA)-based quantitative proteomic technology at 96 h post-infection (hpi). Proteins with P-values < 0.05 and fold change> 1.5 or <0.67 were considered as proteins with differential abundance (PDAs). Results: A total of 28 down-regulated PDAs and 407 up-regulated PDAs were identified at 96 hpi, including RhoC, TM4SFs and LPCAT1, which could be associated with the maintenance and repair of lung homeostasis. Gene Ontology (GO) annotation and K

ORGANISM(S): Canis Lupus Familiaris

SUBMITTER: Xingquan Zhu

PROVIDER: PXD041668 | iProX | Thu Apr 20 00:00:00 BST 2023

REPOSITORIES: iProX

ACCESS DATA

Publications

Proteomic change in the upper lobe of the left lung of Beagle dogs at the lung migration stage of Toxocara canis infection.

Zheng Wen-Bin WB Qiu Hui-Jie HJ Xiao Han-Dan HD Zou Yang Y Zhu Xing-Quan XQ

Parasites & vectors 20240509 1

<h4>Background</h4>Toxocara canis is considered one of the most neglected parasitic zoonoses and threatens the health of millions of people worldwide with a predilection for pediatric and adolescent populations in impoverished communities. Exploring the invasion and developmental mechanisms associated with T. canis infection in its definitive canine hosts will help to better control zoonotic toxocariasis.<h4>Methods</h4>Proteomic changes in samples from the upper lobe of the left lung of Beagle ...[more]

PMID: 38725025

Similar Datasets

Project description:To combat virus infections, which are major human killers, a deeper understanding of how viruses reprogram their hosts to create optimal production of progeny is needed. Most knowledge on the regulation of cellular gene expression during adenovirus infection is derived from studies of mRNA expression. Here, we investigated the changes in cellular protein expression during the late phase of adenovirus type 2 (Ad2) infection of primary human cells by stable isotope labeling in cell culture (SILAC) with subsequent liquid chromatography-high resolution tandem mass spectrometric (LC-MS/MS) analysis using a Q-Exactive Orbitrap instrument. Cells were in-depth evaluated 24 and 36 hours post infection (hpi) and two biological replicates were investigated using swapped labeling. In total, 2648 and 2394 proteins were quantified at 24 and 36 hpi, respectively. Among them, 659 and 645 were deregulated more than 1.6-fold at the two time points, and approximately 80% of them had sustained regulation from 24 to 36 hpi. For the deregulated genes the change in protein expression was compared with that of RNA expression using cDNA sequencing data from the same time points of infection. The comparison revealed a surprisingly low correlation (r = 0.3). Consistency between changes in RNA and protein expression was observed for approximately 30% of the genes, whereas a majority showed either divergent or opposite expression profiles. The deregulated proteins were subjected to pathway and biological function analysis using bioinformatics tools. A surprising observation was that proteins related to carbohydrate metabolism were up-regulated at the protein level but unchanged at the RNA level whereas histone proteins were down-regulated at the protein level but up-regulated at the RNA level. Our results show that regulation of cellular gene expression in the response to viral infection is more complex than hitherto believed and several examples of posttranscriptional regulation of gene expression were observed. The results contribute to our understanding of host cell gene regulation during infection at a deeper level.

Dataset Information

Proteome alterations in the lung of Beagle dogs during the lung migration stage of Toxocara canis infection

Publications

Proteomic change in the upper lobe of the left lung of Beagle dogs at the lung migration stage of Toxocara canis infection.

Similar Datasets

OmicsDI is part of the ELIXIR infrastructure