Dataset Information

Proteomic analysis of TMT of sea urchin(Strongylocentrotus intermedius) under high temperature stress

ABSTRACT: In order to investigate the protein expression of S. intermedius under high temperature stress. The study was conducted with the high-temperature resistant strain of S. intermedius and the control group of S. intermedius.

ORGANISM(S): Strongylocentrotus Intermedius

SUBMITTER: Jun Ding

PROVIDER: PXD043884 | iProX | Tue Jul 18 00:00:00 BST 2023

REPOSITORIES: iProX

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Publications

TMT-based proteomics analysis of sea urchin (Strongylocentrotus intermedius) under high temperature stress.

Hao Pengfei P Han Lingshu L Wu Yanglei Y Wang Yongjie Y Ruan Shuchao S Liu Ziyu Z Zhang Weijie W Ding Jun J

Comparative biochemistry and physiology. Part D, Genomics & proteomics 20231225

In the context of global warming and continuous high temperatures in the northern part of China in summer, the mortality rate of Strongylocentrotus intermedius through the summer reaches 70-80 %. The protein regulatory mechanism of S. intermedius in response to high temperature stress is still unclear. In order to investigate the protein expression of S. intermedius under high temperature stress, the study was conducted with the high-temperature resistant strain of S. intermedius and the control ...[more]

PMID: 38159402

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Project description:Cell proliferation is achieved by numerous enzyme reactions. Temperature governs the activity of each enzyme, which overall determines the optimal growth temperature. Synthesizing useful chemicals and fuels utilizes only part of the metabolic pathways, especially the central metabolic pathways such as glycolysis and TCA cycle to metabolize glucose. However, the optimal temperature for the activity of the central metabolic pathways is inconclusive whether it is correlated with the optimal temperature for cell proliferation. Here, we found that Corynebacterium glutamicum wild type increased the metabolic activity to consume glucose under anaerobic (oxygen deprived) conditions at 42.5ºC, in which the cell hardly grows under aerobic conditions. Glucose consumption rate was increased by 24% at 42.5ºC compared to that at the optimal growth temperature of 30.0ºC. Transcriptional analysis showed that gapX gene encoding glycelaldehydre-3-phosphate dehydrogenase, glucokinase gene, and a gene involved in glucose uptake were upregulated at 42.5ºC. Production of fermentative lactate was increased by 69% than that at 30.0ºC, whereas succinate production was decreased by 13%. In addition to several glycolytic enzymes, the activity of pyruvate kinase was increased with increasing the temperature, whereas the activity of phosphoenolpyruvate carboxylase in anapleotic pathway leading to succinate synthesis was decreased. However, a metabolically engineered succinate over-producing strain, in which lactate production was shut off and pyruvate carboxylase encoding gene in another anapleotic pathway was overexpressed, produced 34% higher succinate at 42.5ºC than that at 30.0ºC with increased glucose consumption. This study provides the evidence that the optimal reaction temperature for production of fermentative products can be set beyond upper limit of growth temperature in C. glutamicum, and hence it could be applicable for producing various chemicals and fuels in C. glutamicum under anaerobic conditions and non-growing cell reactions in other microorganisms.

Dataset Information

Proteomic analysis of TMT of sea urchin(Strongylocentrotus intermedius) under high temperature stress

Publications

TMT-based proteomics analysis of sea urchin (Strongylocentrotus intermedius) under high temperature stress.

Similar Datasets

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