Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

LC-MS/MS identification of RPL22 interaction proteins

ABSTRACT: Proteins obtained in Co-IP were subjected to SDS-PAGE gel electrophoresis and Coomassie brilliant blue staining. After decolorization, the SDS-PAGE gel was sent to the mass spectrometry platform of the Institute of Biophysics, Chinese Academy of Sciences for subsequent processing and mass spectrometry detection.

ORGANISM(S): Homo Sapiens

SUBMITTER: Xiaoyu Jiang

PROVIDER: PXD044052 | iProX | Mon Jul 24 00:00:00 BST 2023

REPOSITORIES: iProX

ACCESS DATA

Json Xml

Similar Datasets

Identification of a novel Glis3-348aa protein in MIN6 cells by LC-MS

Project description:To identify the novel protein encoded by circGlis3, total protein lysates were extracted from MIN6 cells transfected with circGlis3 plasmid or empty vector and separated by SDS-PAGE, followed by Coomassie brilliant blue staining. The differential gel bands near 35 kDa were excised and subjected to mass spectrometry.

2023-11-02 | PXD037447 | Pride

Aurora A phosphorylates PKM2 at T45.

Project description:Flag-PKM2 was expressed in H1299 cells. Then the H1299 cells were treated with DMSO or 100nM MLN8237. Then, cell lysate was incubated with anti-Flag M2-agarose. Elutes were separated with 10% SDS-PAGE. The gel was stained with Coomassie Brilliant Blue. Visualized bands were excised and subjected to LC-MS/MS analysis. Then the phosphorylation sites of PKM2 were identified.

2023-03-11 | PXD036575 | Pride

Identification of MGF360-13L interacting proteins by LC-MS/MS in iPAM

Project description:Co-immunoprecipitation assay was investigated and subjected to SDS-PAGE. The gels were stained by Coomassie Brilliant Blue G-250 and sent to Novogene (Beijing, China) for LC-MS/MS analysis to identified the potential interaction protein between MGF360-13L and host protein.

2022-11-16 | PXD038172 | iProX

Identification of novel KRT32-associated proteins in primary keratinocytes cells lysate by GST-KRT32 pulldown assays coupled with mass spectrometry.

Project description:GST-KRT32 and its interacting proteins in primary keratinocytes cells were enriched by pull-down using glutathione-Sepharose beads. Then, KRT32-interacting proteins were identified by mass spectrum. Proteins obtained in Pulldown were subjected to SDS-PAGE gel electrophoresis and silver staining. The SDS-PAGE gel was sent to the mass spectrometry platform of the Jingjie PTM Biolab for subsequent processing and mass spectrometry detection.

2024-06-17 | PXD050141 | Pride

Strategies to enhance the extraction of electrophoretically separated proteins from polyacrylamide gels and their application to mass spectrometry analyses (C4PR_LIV)

Project description:Polyacrylamide gel electrophoresis (PAGE) is a powerful technique for separating proteins extracted from complex biological samples. Unfortunately, the difficulty of recovering intact proteins in high yield from polyacrylamide matrices often limits further analyses. We discovered that staining proteins with Coomassie brilliant blue (CBB) immediately after electrophoresis improves extraction efficiency. Post-staining, proteins widely varying in molecular weight were recovered at high efficiency with a 10 minute procedure that did not employ a surfactant. High recoveries were also obtained from dried, archived gels. Native-PAGE separated protein complexes larger than 400 kDa were recovered in an alternative procedure that substituted the mild detergent octyl-β-D glucopyranoside for CBB. Recovered proteins retained their native structure and were amenable to structural analysis by native mass spectrometry. The established workflows facilitate in-depth proteomic and protein structure analyses as well as the purification, storage, and transport of protein samples.

2020-07-13 | PXD010592 | Pride

Yersinia enterocolitica SOR17 - The RNA chaperone Hfq impacts growth, metabolism and production of virulence factors in Yersinia enterocolitica

Project description:We work with the bacterium Yersinia enterocolitica, a gastrointestinal pathogen. In this study we characterized a bacterial mutant strain called SOR17. We compared the protein extracts of SOR17 with that of the parental strain JB580v. Bacteria were grown in triplicate for 5 h at 37°C, and following bacterial disruption by French press, proteins were fractionated into soluble or membrane proteins. We also compared total protein extracts of bacteria grown for 16 h at 27°C. Two-dimensional electrophoresis were performed and proteins were stained with Coomassie brilliant blue. Spots whose abundance changed more than 2 fold were excised and sent to mass spectrometry for identification by either MALDI-TOF PMF or LC-MS/MS.

2014-04-16 | PXD000475 | Pride

Identification of the potential regulators of G6PD activity in different cell cycle

Project description:Flag-G6PD was expressed in HeLa cells. Then the HeLa cells were synchronized with a double thymidine block procedure. Briefly, the exponentially growing HeLa cells were maintained with 2 mM thymidine for 18 h, followed by a release of 9 h in fresh medium, and then cells were re-cultured in 2 mM thymidine for additional 15 h. These interphase cells were harvested. After a release of 8.5 h in fresh medium, the cells entered into M phase. Mitotic cells were harvested by mitotic shake-off. Then, cell lysate was incubated with anti-Flag M2-agarose. Elutes were separated with 10% SDS-PAGE. The gel was stained with Coomassie Brilliant Blue. Visualized bands were excised and subjected to LC-MS/MS analysis.

2023-05-18 | PXD033829 | Pride

Identification of proteins interacted with EphA2 in nasopharyngeal carcinoma cells by LC-MS / MS

Project description:To search proteins that interact with EphA2, EphA2 interactors were coimmunoprecipitated with anti-EphA2 antibody from 5-8F NPC cell extracts, separated on SDS-PAGE and stained with Coomassie blue , and non-immune IgG antibody instead of anti-EphA2 antibody served as control. All bands were excised, and subjected to in gel trypsin digestion and liquid chromatography and high-throughput mass spectrometry (LC-MS/MS) analysis.As a result, a total of1352 proteins including ANXA1 and Cbl were identified .

2021-09-08 | PXD015242 | Pride

Thylakoids of Picea abies - The unique photosynthetic apparatus of Pinaceae - Analysis of photosynthetic complexes in Norway spruce (Piceaabies)

Project description:Analysis of the subunits composition of the thylakoids protein complexes in Picea abies (Norway spruce) by means of two-dimensional large-pore Blue-Native/sodium dodecyl sulfate-polyacrylamide gel electrophoresis (2D lpBN/SDS-PAGE) and in-gel tryptic digestion of single spots.

2019-04-02 | PXD010071 | Pride

Aspirin Acetylates Thioredoxin Reductase 1 (isoform 4)

Project description:Thioredoxin Reductase 1 (isoform 4) was incubated with aspirin for 1 hour at 37 C followed by SDS PAGE. Samples were picked from SDS PAGE gel, digested with trypsin, and sent for LC-MS/MS.

2022-02-17 | PXD027882 | Pride

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data