Proteome changes of Aspergillus flavus before and after adding essential oil treatment
Ontology highlight
ABSTRACT:
ORGANISM(S): Aspergillus Flavus Nrrl3357
SUBMITTER: 
Qing Zhang
PROVIDER: PXD046563 | iProX | Wed Nov 01 00:00:00 GMT 2023
REPOSITORIES: iProX
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Project description:The purpose of this study was to explore the mechanism of aerobic decay of whole-plant corn silage and the effect of Neolamarckia cadamba essential oil on aerobic stability of whole-plant corn silage. Firstly, the dynamic changes of temperature, microbial community and metabolite content after aerobic exposure of whole-plant corn silage were determined, and the main microbial species and mechanism leading to aerobic spoilage of whole-plant corn silage were analyzed. The N. cadamba essential oil was extracted from fresh N. cadamba leaves by steam distillation, and the minimal inhibitory concentration, antibacterial stability and bacteriostatic mechanism of N. cadamba essential oil against undesirable microorganisms in whole-plant corn silage were determined. According to the minimum inhibitory concentration of N. cadamba essential oil on undesirable microorganisms in silage, N. cadamba essential oil was added to whole-plant corn silage to explore the effect of N. cadamba essential oil on the aerobic stability of whole-plant corn silage.
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Project description:The present study aimed to investigate the antibacterial effect and mechanism of a carvacrol-rich Oregano essential oil (OEO) on the inactivation of Staphylococcus aureus. A label-free proteomic analysis was applied to analyze the regulatory networks of S. aureus in response to 1/2 MIC OEO-treatment stress. These findings deepened our understanding regarding S. aureus survival and metabolism responses to the OEO treatment and suggested that the carvacrol-rich OEO could be used in food production environments to effectively control S. aureus.
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Project description:Background: Frankincense (Ru Xiang) and sandalwood (Tan Xiang) are ingredients used in traditional Chinese medicine, and have been recognized as cancer preventive and therapeutic agents. Hydrodistillation of frankincense gum resins and sandalwood heartwood to prepare essential oils is a method to extract biologically active ingredients from these plant-derived products. This study was designed to differentiate frankincense (Boswellia carterii) and sandalwood (Santalum album) induced anti-proliferative and pro-apoptotic activities in cultured human bladder cancer cells. Methods: Frankincense and sandalwood essential oils-mediated cytotoxicity was studied in established human bladder cancer J82 cells and immortalized normal human bladder urothelial UROtsa cells using a colorimetric assay. Essential oils-activated gene expression and pathway activation in human bladder cancer J82 cells were identified using high density microarray and bioinformatics techniques. Results: Human bladder cancer cells were more sensitive to immortalized normal bladder cells with suppressed viability following frankincense essential oil exposure. In contrast, both cancerous and normal bladder cells responded to sandalwood essential with similar levels of cytotoxicity. Based on microarray and bioinformatics analyses, genes responsible for suppressing biological processes and apoptosis were induced in J82 cells by both essential oils. Although both frankincense and sandalwood essential oils activated common ontologies and canonical pathways leading to suppressed J82 cell viability and apoptosis, each essential oil had unique property on these cells. For example, heat shock proteins and histone core were ongologies regulated by frankincense essential oil, whereas transcription regulation and G-protein couple receptor were ontologies unique to sandalwood essential oil treatment. In addition, NRF-2 mediated oxidative stress was implicated as the primary cause of frankincense essential oil-induced J82 cell death; in contrast, DNA damage and cell cycle arrest might be attributed to sandalwood essential oil-mediated cytotoxicity. Conclusion: Based on cell biology and comprehensive gene expression analysis, our results provide a preliminary, yet focused characterization of genetic responses to frankincense and sandalwood essential oils with respect to their proposed anti-neoplastic properties. Modern biomedical technologies are powerful tools to study biological responses following treatments with traditional Chinese medicine, which always consist of complex chemical constituents. To differentiate mechanisms of frankincense and sandalwood essential oils induced cytotoxicty in bladder cancer J82 cells, time-dependent transcriptoms expression was performed in cultured cells following essential oils treatments
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Project description:Background: Frankincense (Ru Xiang) and sandalwood (Tan Xiang) are ingredients used in traditional Chinese medicine, and have been recognized as cancer preventive and therapeutic agents. Hydrodistillation of frankincense gum resins and sandalwood heartwood to prepare essential oils is a method to extract biologically active ingredients from these plant-derived products. This study was designed to differentiate frankincense (Boswellia carterii) and sandalwood (Santalum album) induced anti-proliferative and pro-apoptotic activities in cultured human bladder cancer cells. Methods: Frankincense and sandalwood essential oils-mediated cytotoxicity was studied in established human bladder cancer J82 cells and immortalized normal human bladder urothelial UROtsa cells using a colorimetric assay. Essential oils-activated gene expression and pathway activation in human bladder cancer J82 cells were identified using high density microarray and bioinformatics techniques. Results: Human bladder cancer cells were more sensitive to immortalized normal bladder cells with suppressed viability following frankincense essential oil exposure. In contrast, both cancerous and normal bladder cells responded to sandalwood essential with similar levels of cytotoxicity. Based on microarray and bioinformatics analyses, genes responsible for suppressing biological processes and apoptosis were induced in J82 cells by both essential oils. Although both frankincense and sandalwood essential oils activated common ontologies and canonical pathways leading to suppressed J82 cell viability and apoptosis, each essential oil had unique property on these cells. For example, heat shock proteins and histone core were ongologies regulated by frankincense essential oil, whereas transcription regulation and G-protein couple receptor were ontologies unique to sandalwood essential oil treatment. In addition, NRF-2 mediated oxidative stress was implicated as the primary cause of frankincense essential oil-induced J82 cell death; in contrast, DNA damage and cell cycle arrest might be attributed to sandalwood essential oil-mediated cytotoxicity. Conclusion: Based on cell biology and comprehensive gene expression analysis, our results provide a preliminary, yet focused characterization of genetic responses to frankincense and sandalwood essential oils with respect to their proposed anti-neoplastic properties. Modern biomedical technologies are powerful tools to study biological responses following treatments with traditional Chinese medicine, which always consist of complex chemical constituents.
2014-06-10 | GSE53171 | GEO
Project description:Transcriptome analysis plays a central role in elucidating the complexity of gene expression regulation in Escherichia coli. By analyzing the transcriptomics of E. coli treated with water, acetone, and Cinnamomum camphora essential oil, the inhibitory mechanism of the essential oil on the human intestinal microbe was studied. The results showed that the inhibitory effect of the essential oil on E. coli increased with an increase in concentration; 1/4 minimum inhibitory concentration was the reaction equilibrium point. RNAseq transcriptomic comparison indicated that the essential oil inhibited the growth of E. coli by inhibiting the metabolism, chemotaxis, and some resistance reactions of them, while E. coli maintained its life activities by enhancing its resistance reactions. These results are of great importance to the study of the medical use of C. camphor essential oil and gene regulation in E. coli under stress conditions.
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Project description:RNAseq analysis of antibacterial mechanism of Cinnamomum camphora essential oil against Escherichia coli
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Project description:The purpose of the present study was to establish at the molecular level, a genetic profile of the biological activity of essential sandalwood oil from Santalum album, produced under standardized conditions. The model system under investigation is based on living human skin explants. This protocol permits to compare ex vivo the effects of SO at the histological level as well as the changes of gene expression as a function of SO concentration and duration of treatment. In the present report, Sandalwood oil (S. album) was applied to living human skin explants to evaluate the sustained activity of the oil through gene expression profiling. Transcriptomic analysis showed a number of metabolic pathways and biological activities triggered by treatments previously described for aloe, curcumin and luteolin. No inflammatory responses accompanied the beneficial activities. The most intriguing impact was the natural effect on the retinoic acid (vitamin A) metabolism. Furthermore, we found that mechanisms normally acting in more specialized cell types such as immune cells, adipocytes, nerve cells, or hair follicles were activated in human skin explants in response to sandalwood oil. Thus, the oil may have the potential of further beneficial activities such as its antibacterial activity among other protective mechanisms. These results corroborate at a molecular level the rationale for clinical studies with sandalwood oil and support recent studies of patients with eczema (atopic dermatitis) or acne, which is often accompanied by bacterial growth on skin of Propionibacterium acnes.
2017-11-10 | GSE106734 | GEO
Project description:Analysis of the effect of C. sativum (coriander) essential oil on the human genome with toxicological purposes. Total RNA was obtained from Hela Cells (CCL-2) treated with the essential oil at IC30 (inhibition concentration).
2014-04-03 | E-GEOD-56431 | biostudies-arrayexpress
Project description:Interventions: Others(Aroma Essential Oil Inhalation) : In this study, data of the control group were collected and data of the experimental group were collected to prevent the spread of the experimental treatment by the time difference design of the non - equality control group. In the experimental group, 3 drops (1 drop = 0.05cc) of aroma essential oil blended with 2:1 lavender and ylang ylang on the 2nd and 3rd day of hospitalization were inhaled by deep breathing, 30 cm, and the control group was provided with general nursing care without any treatment.
Primary outcome(s): Quality of sleep
Primary Purpose : Supportive Care, Intervention Model : Parallel, Blinding/Masking : Single, Blinding Target : Subject, Allocation : Non-RCT
| 2511433 | ecrin-mdr-crc