Project description:TAF4 directed immunoprecipitation of the the Pre-initiation complex from mouse embryonic stem cells with or without depletion of TATA-box binding protein (TBP).

2022-05-27 | MSV000089562 | MassIVE

Mouse embryonic stem cell phosphoproteome

Project description:Erk-driven differentiation of mouse embryonic stem cells

2019-10-22 | PXD008964 | Pride

AP-MS of EPOP in mouse embryonic stem cells

Project description:A Flag-tagged version of murine EPOP was expressed in mouse embryonic stem cells (ESCs) at near to endogenous levels and purified using an anti-FLAG affinity matrix.

2016-12-13 | PXD005082 | Pride

AP-MS of Elongin B in mouse embryonic stem cells

Project description:A Flag-tagged version of murine Elongin B was expressed in mouse embryonic stem cells (ESCs) at near to endogenous levels and purified using an anti-FLAG affinity matrix.

2016-12-13 | PXD005081 | Pride

The FGF4-dependent phosphoproteome in mouse embryonic stem cells

Project description:Protein kinase signalling is a major mechanism by which embryonic stem cell pluripotency and differentiation is controlled. However, the pathways and components that regulate embryonic stem cell identity have not been systematically defined. Here, we employ FGF4 signalling as a model system to investigate phosphoproteome dynamics in differentiating mouse embryonic stem cells. We report identification and quantitation of more than 10,000 phosphopeptides, of which hundreds of phosphophoylation sites are regulated more than 2-fold by acute FGF4 stimulation. We hypothesise that phosphorylation sites in this dataset are relevant for regulating the transition of mouse embryonic stem cells from pluripotency towards lineage specific differentiation.

2020-02-24 | PXD012069 | Pride

Characterisation of Myst2 and Niam interactomes in mouse embryonic stem cells

Project description:Here we undertake the characterisation of Myst2 protein interactions in mouse embryonic stem cells by affinity purification and mass spectrometry using a Myst2-FTAP tagged cell line. This protein interaction study confirms that in mouse embryonic stem cells Myst2 is part of H3 and H4 histone acetylation complexes similar to those described in somatic cells. We identify a novel Myst2- associated protein, the tumour suppressor protein Niam (Nuclear Interactor of Arf and Mdm2), and show that it also interacts with subunits of Myst2 H4 HAT complexes. Human NIAM is involved in chromosome segregation, p53 regulation and cell proliferation in somatic cells, but its role in embryonic stem cells is unknown. We describe the first Niam embryonic stem cell interactome, which includes proteins with roles in DNA replication and repair, transcription, splicing and ribosome biogenesis. Many of Myst2 and Niam binding partners are required for correct embryonic development, implicating Myst2 and NIAM in the cooperative regulation of this process and suggesting a novel role for Niam in embryonic biology. The data provides a useful resource for exploring Myst2 and Niam essential cellular functions and should contribute to deeper understanding of organism development and survival as well as the development of cancer.

2017-08-22 | PXD005987 | Pride

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