Proteomics

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EIF3H-interactome in Colorectal Cancer cell line (HCT116)

ABSTRACT: To elucidate the molecular mechanism and search for EIF3H substrate(s) of EIF3H-mediated CRC progression, we performed immune-affinity purification and resolved the binding partners utilizing mass spectrometry. HCT116 cells transfected with empty vector or Flag-tagged EIF3H and then immunoprecipitated using anti-Flag M2 beads.The purified EIF3H protein complex was subjected to mass spectrometry analysis.

ORGANISM(S): Homo Sapiens

SUBMITTER: Monghong Lee  

PROVIDER: PXD048151 | iProX | Tue Dec 26 00:00:00 GMT 2023

REPOSITORIES: iProX

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Publications

The EIF3H-HAX1 axis increases RAF-MEK-ERK signaling activity to promote colorectal cancer progression.

Jin Huilin H   Huang Xiaoling X   Pan Qihao Q   Ma Ning N   Xie Xiaoshan X   Wei Yue Y   Yu Fenghai F   Wen Weijie W   Zhang Boyu B   Zhang Peng P   Chen Xijie X   Wang Jie J   Liu Ran-Yi RY   Lin Junzhong J   Meng Xiangqi X   Lee Mong-Hong MH  

Nature communications 20240321 1

Eukaryotic initiation translation factor 3 subunit h (EIF3H) plays critical roles in regulating translational initiation and predicts poor cancer prognosis, but the mechanism underlying EIF3H tumorigenesis remains to be further elucidated. Here, we report that EIF3H is overexpressed in colorectal cancer (CRC) and correlates with poor prognosis. Conditional Eif3h deletion suppresses colorectal tumorigenesis in AOM/DSS model. Mechanistically, EIF3H functions as a deubiquitinase for HAX1 and stabil  ...[more]

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