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Proteome of synthetic ribosomal 40S subunit

ABSTRACT: In this study, we present the first methodology (to the best of our knowledge) enabling reconstruction of the eukaryotic 40S ribosome subunit with completely customized r-proteins, referred to as “Ribo-swap” (Figure 1). In Ribo-swap, a designer platform yeast was developed through relocating the 56 genes encoding the whole set of 32 40S r-proteins from native genome loci to a synthetic gene cluster, which could be further swapped by another one encoding customer-designed 40S r-proteins to reconstruct customer 40S ribosome subunits in yeast.

ORGANISM(S): Saccharomyces Cerevisiae

SUBMITTER: Guanghou Zhao

PROVIDER: PXD054321 | iProX | Sat Jul 27 00:00:00 BST 2024

REPOSITORIES: iProX

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