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SWATH-MS in TCTP-deficient MPNST cells

ABSTRACT: EF1A2-binding proteins in TCTP-deficient MPNST cells were identified by immuno-precipitation using anti-FLAG antibody and SWATH. To verify whether the change of the amount of EF1A2-binding proteins caused the cellular expression change, the proteins expressed in sNF96.2 MPNST cells treated with TCTP-siRNA were profiled by SWATH-MS.

ORGANISM(S): Homo Sapiens (human)

SUBMITTER: Norie Araki

PROVIDER: PXD007606 | JPOST Repository | Mon Nov 05 00:00:00 GMT 2018

REPOSITORIES: jPOST

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Dataset's files

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			Action	DRS
	Data20170315_EF1A2_CONTsi_1.wiff	Wiff
	Data20170315_EF1A2_CONTsi_1.wiff.scan	Wiff
	Data20170315_EF1A2_CONTsi_2.wiff	Wiff
	Data20170315_EF1A2_CONTsi_2.wiff.scan	Wiff
	Data20170315_EF1A2_CONTsi_3.wiff	Wiff

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Publications

Identification of a Specific Translational Machinery via TCTP-EF1A2 Interaction Regulating NF1-associated Tumor Growth by Affinity Purification and Data-independent Mass Spectrometry Acquisition (AP-DIA).

Kobayashi Daiki D Tokuda Takaho T Sato Kyosuke K Okanishi Hiroki H Nagayama Megumi M Hirayama-Kurogi Mio M Ohtsuki Sumio S Araki Norie N

Molecular & cellular proteomics : MCP 20181031 2

Neurofibromatosis type 1 (NF1) is an autosomal dominant disease that predisposes individuals to developing benign neurofibromas and malignant peripheral nerve sheath tumors (MPNST). The mechanism of NF1-tumorigenesis or the curatives have not been established. Using unique trascriptome and proteome integration method, iPEACH (1), we previously identified translationally controlled tumor protein (TCTP) as a novel biological target for NF1-associated tumors (2). Here, we identified specific TCTP-i ...[more]

PMID: 30381327

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SWATH-MS in EF1A1- and EF1A2-deficient Schwann and MPNST cells

Project description:The proteins expressed in normal human Schwann and sNF96.2 MPNST cells treated with EF1A1- and EF1A2-siRNA were profiled by SWATH-MS.

2018-11-05 | PXD007603 | JPOST Repository

EF1A2-binding proteins in TCTP-deficient MPNST cells

Project description:EF1A2-binding proteins in TCTP-deficient MPNST cells were identified by immuno-precipitation using anti-FLAG antibody and SWATH.

2018-11-05 | PXD007605 | JPOST Repository

TCTP interactome analysis by AP-SWATH

Project description:TCTP binding proteins were identified by immunoprecipitation using anti-FLAG antibody and SWATH acquisition.

2018-11-05 | PXD007578 | JPOST Repository

JPST000107 TCTP interactome analysis by AP-SWATH [Reanalysis: JPST000107]

Project description:TCTP binding proteins were identified by immunoprecipitation using anti-FLAG antibody and SWATH acquisition. [Original project description]

2023-04-01 | RPXD034324 | JPOST Repository

TCTP is an androgen-regulated gene implicated in prostate cancer

Project description:TCTP has been implicated in a plethora of important cellular processes related to cell growth, cell cycle progression, malignant transformation and inhibition of apoptosis. In addition to these intracellular functions, TCTP has extracellular functions and plays an important role in immune cells. TCTP expression was previously shown to be deregulated in prostate cancer, but its function in prostate cancer cells is largely unknown. Here we show that TCTP expression is regulated by androgens in LNCaP prostate cancer cells in vitro as well as human prostate cancer xenografts in vivo. Knockdown of TCTP reduced colony formation and increased apoptosis in LNCaP cells, implicating it as an important factor for prostate cancer cell growth. Global gene expression profiling in TCTP knockdown LNCaP cells showed that several interferon regulated genes are regulated by TCTP, suggesting that it may have a role in regulating immune function in prostate cancer. In addition, recombinant TCTP treatment increased colony formation in LNCaP cells suggesting that secreted TCTP may function as a proliferative factor in prostate cancer. These results suggest that TCTP may have a role in prostate cancer development. A total of 6 samples were analyzed. These consisted of three biological replicates for each treatment. The samples transfected with siRNA against Luciferase (siLuc) served as controls.

2013-10-23 | E-GEOD-47471 | biostudies-arrayexpress

TCTP is an androgen-regulated gene implicated in prostate cancer

2013-10-23 | GSE47471 | GEO

TCTP participate in hepatic metabolism with regulate the gene expression involved in insulin resistance

Project description:The translationally controlled tumor protein (TCTP) has various cellular functions and molecular interactions, many related to its growth promoting and antiapoptotic properties. Recently, TCTP highly increased expression in high-fat diet-fed mice with insulin-resistant states. Despite TCTP is a multifunction protein, but its role in liver metabolism is still unknown. Here, we investigated the function and mechanism of TCTP in HepG2 cells. Knock-down the TCTP regulated 287 differentially expressed genes and these genes are highly associated with the cellular apoptosis and stress, NF-kB, Glycolysis/Gluconeogenesis, insulin resistance. TCTP deletion selectively downregulated the expression of PCK1 and G6PC in glucose metabolism. Furthermore, TCTP regulated the alternative splicing of genes enriched in phospholipid biosynthetic process, and glycerophospholipid metabolism. Our results implied that TCTP regulates liver cell transcriptome at both the transcriptional and alternative splicing levels. The TCTP regulatory network predicts the biological functions of TCTP in glycometabolism and lipid metabolism, which may be associated with liver metabolism and induces fatty liver disease through enhanced insulin resistant signaling.

2021-01-13 | GSE143409 | GEO

knockout-Transcriptomic analyses approach to identify genes associated with organ growth downstream TCTP in Arabidopsis

Project description:ra09-02_tctp - knockout - Identification of effector genes that act downstream of AtTCTP, using knockout ( tctp-2), RNAi expressing line (35S::RNAi-AtTCTP) and over expressing line (35S::AtTCTP) - transcriptome analysis in leaf of tctp-2 knock out versus wild type Keywords: gene knock out

2011-03-17 | GSE19847 | GEO

TCTP-interacting proteins in normal and tumor Schwann cells

Project description:Comparison of TCTP-interacting proteins in between sNF96.2 MPNST cells (NF1-associated malignant tumor cells) and human Schwann cells

2018-11-05 | PXD010662 | JPOST Repository

knockout-Transcriptomic analyses approach to identify genes associated with organ growth downstream TCTP in Arabidopsis

2011-03-17 | E-GEOD-19847 | biostudies-arrayexpress