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Affinity purification to find Arabidopsis TSN1 and TSN2 interacting proteins

ABSTRACT: As the first step towards a better understanding of the regulation and molecular function of SGs and PBs in plants, we performed affinity purification and mass spectrometry of proteins constituting these mRNP complexes in Arabidopsis. Since TSN has been ascribed a scaffolding role in recruiting other proteins to the stress-induced cytoplasmic mRNP foci, we used two Arabidopsis TSN homologs, TSN1 and TSN2, as baits to purify plant mRNP complex-associated proteins. Our experiments revealed that direct homologs of key components of yeast and animal SGs and PBs are bound to TSN in plants. Interestingly, we also found a group of TSN-interacting proteins that appear to be novel or plant-specific components of mRNP complexes.

ORGANISM(S): Arabidopsis Thaliana (mouse-ear Cress)

SUBMITTER: Peter V. Bozhkov and Emilio Gutierrez-Beltran

PROVIDER: PXD007702 | JPOST Repository | Sat Feb 25 00:00:00 GMT 2023

REPOSITORIES: jPOST

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Project description:Ouyang2014 - photomorphogenic UV-B signalling network This model is described in the article: Coordinated photomorphogenic UV-B signaling network captured by mathematical modeling. Ouyang X, Huang X, Jin X, Chen Z, Yang P, Ge H, Li S, Deng XW. Proc. Natl. Acad. Sci. U.S.A. 2014 Aug; 111(31): 11539-11544 Abstract: Long-wavelength and low-fluence UV-B light is an informational signal known to induce photomorphogenic development in plants. Using the model plant Arabidopsis thaliana, a variety of factors involved in UV-B-specific signaling have been experimentally characterized over the past decade, including the UV-B light receptor UV resistance locus 8; the positive regulators constitutive photomorphogenesis 1 and elongated hypocotyl 5; and the negative regulators cullin4, repressor of UV-B photomorphogenesis 1 (RUP1), and RUP2. Individual genetic and molecular studies have revealed that these proteins function in either positive or negative regulatory capacities for the sufficient and balanced transduction of photomorphogenic UV-B signal. Less is known, however, regarding how these signaling events are systematically linked. In our study, we use a systems biology approach to investigate the dynamic behaviors and correlations of multiple signaling components involved in Arabidopsis UV-B-induced photomorphogenesis. We define a mathematical representation of photomorphogenic UV-B signaling at a temporal scale. Supplemented with experimental validation, our computational modeling demonstrates the functional interaction that occurs among different protein complexes in early and prolonged response to photomorphogenic UV-B. This model is hosted on BioModels Database and identified by: BIOMD0000000545. To cite BioModels Database, please use: BioModels Database: An enhanced, curated and annotated resource for published quantitative kinetic models. To the extent possible under law, all copyright and related or neighbouring rights to this encoded model have been dedicated to the public domain worldwide. Please refer to CC0 Public Domain Dedication for more information.

			Action	DRS
	All%20result%20HeatMap%20.xlsb	Xls
	GFP%20HeatMap.xlsb	Xls
	TSN1%20HeatMap.xlsb	Xls
	TSN2%20HeatMap%20.xlsb	Xls

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Affinity purification to find Arabidopsis TSN1 and TSN2 interacting proteins

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