Dataset Information

Retinal-cysteine adduct in Opn5L1

ABSTRACT: Targeted analysis of retinal-cysteine adduct in Opsin 5 like-1 (Opn5L1) protein Chicken Opn5L1 E177K/Q192K mutant was expressed in HEK293T, regenerated by all-trans-retinal, solubilized by dodecyl maltoside, and purified using Rho1D4 antibody-conjugated Sephalose. Semi-quantitative targeted analyses of trypsinized Opn5L1 with or without light irradiation were carried out using reverse phase LC-ESI-MSn to detect light-dependent formation of retinal-cysteine adduct. The results were analyzed by Mass++, mMass and Igor Pro softwares.

ORGANISM(S): Gallus Gallus

SUBMITTER: Yoshinori Shichida

PROVIDER: PXD008890 | JPOST Repository | Thu Feb 15 00:00:00 GMT 2018

REPOSITORIES: jPOST

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Publications

Opn5L1 is a retinal receptor that behaves as a reverse and self-regenerating photoreceptor.

Sato Keita K Yamashita Takahiro T Ohuchi Hideyo H Takeuchi Atsuko A Gotoh Hitoshi H Ono Katsuhiko K Mizuno Misao M Mizutani Yasuhisa Y Tomonari Sayuri S Sakai Kazumi K Imamoto Yasushi Y Wada Akimori A Shichida Yoshinori Y

Nature communications 20180328 1

Most opsins are G protein-coupled receptors that utilize retinal both as a ligand and as a chromophore. Opsins' main established mechanism is light-triggered activation through retinal 11-cis-to-all-trans photoisomerization. Here we report a vertebrate non-visual opsin that functions as a Gi-coupled retinal receptor that is deactivated by light and can thermally self-regenerate. This opsin, Opn5L1, binds exclusively to all-trans-retinal. More interestingly, the light-induced deactivation through ...[more]

PMID: 29593298

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	Dark_MS2_mMass%20Report.html	Other
	Dark_MS2_mMass_spectrum.png	Other
	Dark_MS2_peaklist.mgf	Mgf
	Dark_MS2_spectrum.txt	Txt