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Shotgun proteomics of Mn (II)-oxidizing Serratia marcescens

ABSTRACT: In order to gain insight into how bacteria respond to manganese (II) and relate to oxidation, the shotgun proteomics approach was applied to a potential Mn (II) oxidizing Serratia marcescens strain cultivated in the absence and presence of Mn (II). The LG1 strain, which grew equally well in these two conditions, presented a set of proteins related to the cellular processes vital to its survival and proteins that ensured its good adaptation and tolerance to Mn (II). The multicopper oxidase CueO was identified, indicating its probable participation in the Mn (II) bio-oxidation, but its expression was not modulated by the presence of Mn (II).Through these findings, we reiterate the ability of LG1 to be applied successfully in the bioremediation of Mn (II) environments and the efficiency of the shotgun approach in obtaining the first total shotgun proteome of this specie.

ORGANISM(S): Serratia Marcescens

SUBMITTER: Pollyana Santos Queiroz

PROVIDER: PXD010681 | JPOST Repository | Sat Aug 04 00:00:00 BST 2018

REPOSITORIES: jPOST

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Project description:Haloalkaliphilic microorganisms are double extremophiles functioning optimally at high salinity and pH. Their typical habitats are soda lakes, representing geologically ancient ecosystems which are still widespread on Earth and supposedly harbor relict microbial communities. We compared metabolic features and their genomic determinants in two strains of a single natronophilic species Dethiobacter alkaliphilus, the only cultured representative of “Dethiobacteria” class within Bacillota phylum. The strains of D. alkaliphilus were previously isolated from geographically remote Mongolian and Kenyan soda lakes. The type strain AHT1T was described as a facultatively chemolithoautotrophic sulfidogen reducing or disproportionating sulfur or thiosulfate, while strain Z-1002 was isolated as a chemolithoautotrophic iron reducer. Here, we uncovered iron reducing ability of strain AHT1T, as well as the capability of strain Z-1002 for thiosulfate reduction and anaerobic Fe(II) oxidation. Key catabolic processes sustaining the growth of both strains appeared to fit the geochemical settings of two contrasting natural alkaline environments, sulfur-enriched soda lakes and iron-enriched serpentinites. This assumption was supported by meta-analysis of publicly available Dethiobacterial metagenomes, as well as by the enrichment of a novel phylotype from a deep subsurface non-serpentinizing slightly alkaline water after its amendment with an Fe(III) mineral. Genome analysis of D. alkaliphilus strains revealed that the most probable determinants of iron and sulfur redox transformations in the organism are multiheme c-type cytochromes. Their phylogeny reconstruction showed that sulfur and thiosulfate respiration is most probably provided by evolutionary early forms of unconventional octaheme tetrathionate and sulfite reductases sharing a root with structurally similar group of OmhA/OcwA Fe(III)-reductases. Large sets of other multihemes are likely to provide Fe(III) reduction in both strains. Also, several different, yet phylogenetically related, determinants of anaerobic Fe(II) oxidation were identified in Z-1002 genome, and the oxidation process was further experimentally proven. Considering these results and phylogenetic relatedness of D. alkaliphilus’s sulfur reductases with Fe(III) reducing cytochromes, but not with archetypal bacterial sulfur/thiosulfate reductases, we suggest that sustaining high variation of multiheme cytochromes is an effective adaptive strategy to occupy geochemically contrasting alkaline anaerobic environments. We further propose that sulfur-enriched soda lakes are secondary habitats for D. alkaliphilus comparing to Fe-rich serpentinites, and discuss the evolutionary traits which might occur in prokaryotes on a crucial junction of the biosphere’s history, when intensification of the sulfur cycle outweighed the global significance of the iron cycle.

			Action	DRS
	LG1AMn.mgf	Mgf
	LG1AMn.mzML	Mzml
	LG1AMn.raw	Raw
	LG1BMn.mgf	Mgf
	LG1BMn.mzML	Mzml

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Shotgun proteomics of Mn (II)-oxidizing Serratia marcescens

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