Dataset Information
Mass Spectrometry-compatible Subcellular Fractionation
Ontology highlight
ABSTRACT:
ORGANISM(S): Homo Sapiens (human)
SUBMITTER: Yasushi Ishihama
PROVIDER: PXD013581 | JPOST Repository | Fri Nov 15 00:00:00 GMT 2019
REPOSITORIES: jPOST
Dataset's files
| Action | DRS | |||
|---|---|---|---|---|
| 120427tm_001_Cyt1.RAW | Raw | |||
| 120427tm_001_Cyt1.mgf | Mgf | |||
| 120427tm_002_Cyt2.RAW | Raw | |||
| 120427tm_002_Cyt2.mgf | Mgf | |||
| 120427tm_003_Cyt3.RAW | Raw |
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Publications
Mass Spectrometry-Compatible Subcellular Fractionation for Proteomics.
Journal of proteome research 20191017 1
We found that nuclear envelopes stabilize against surfactants in the presence of ethylene glycol (EG). We, therefore, developed a novel subcellular fractionation approach for proteomics using RIPA buffer containing EG and phase transfer surfactants. This method involves separating the cells into the cytoplasm, organelles, and nucleus, including intermediate filaments without ultracentrifugation. These fractions are directly applicable to sample preparation for shotgun proteomics as they have no ...[more]