Dataset Information

Glycan Markers of Human Stem Cells Assigned with Beam Search Arrays

ABSTRACT: Glycan antigens recognized by monoclonal antibodies have served as stem cell markers. To understand regulation of their biosynthesis and their roles in stem cell behavior precise assignments are required. We have applied state-of-the-art glycan array technologies to compare the glycans bound by five antibodies that recognize carbohydrates on human stem cells. These are: FC10.2, TRA-1-60, TRA-1-81, anti-i and R-10G. Microarray analyses with a panel of sequence-defined glycans corroborate that FC10.2, TRA-1-60, TRA-1-81 recognize the type 1-based backbone sequence, Galβ-3GlcNAcβ-3Galβ-4GlcNAcβ-3Galβ-4GlcNAc, and anti-i, the type 2-analog, Galβ-4GlcNAcβ-3Galβ-4GlcNAcβ-3Galβ-4GlcNAc, and we determine substituents they can accommodate. They differ from R-10G, which requires sulfate. By Beam Search approach, starting with an antigen-positive keratan sulfate polysaccharide, followed by targeted iterative microarray analyses of glycan populations released with keratanases and mass spectrometric monitoring, R-10G is assigned as a unique mono-sulfated type 2 chain, Galβ-4GlcNAc(6S)β-3Galβ-4GlacNAcβ-3Galβ-4GlcNAc. Microarray analyses using newly synthesized glycans corroborate the assignment of this unique determinant raising questions regarding involvement as a ligand in the iPS cell niche.

ORGANISM(S): Cellular Organisms

SUBMITTER: Ten Feizi

PROVIDER: PXD013972 | JPOST Repository | Tue Jun 18 00:00:00 BST 2019

REPOSITORIES: jPOST

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Glycan Markers of Human Stem Cells Assigned with Beam Search Arrays.

Wu Nian N Silva Lisete M LM Liu Yan Y Zhang Yibing Y Gao Chao C Zhang Fuming F Fu Li L Peng Yanfei Y Linhardt Robert R Kawasaki Toshisuke T Mulloy Barbara B Chai Wengang W Feizi Ten T

Molecular & cellular proteomics : MCP 20190715 10

Glycan antigens recognized by monoclonal antibodies have served as stem cell markers. To understand regulation of their biosynthesis and their roles in stem cell behavior precise assignments are required. We have applied state-of-the-art glycan array technologies to compare the glycans bound by five antibodies that recognize carbohydrates on human stem cells. These are: FC10.2, TRA-1-60, TRA-1-81, anti-i and R-10G. Microarray analyses with a panel of sequence-defined glycans corroborate that FC1 ...[more]

PMID: 31308253

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