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Egg-coat degradation

ABSTRACT: Vertebrate oocytes are coated with a special extracellular matrix termed egg-coat or Zona pellucida (ZP). Egg-coat is constituted by several ZP glycoproteins. In chicken fertilization, ejaculated sperm releases enzyme(s) to solubilize the ZP glycoprotein(s) for penetrating the egg-coat. In this study, we analyzed the solubilized ZP glycoprotein fragment. We also analyzed the glycosylation property of its fragment.

ORGANISM(S): Gallus Gallus

SUBMITTER: Tsukasa Matsuda

PROVIDER: PXD013994 | JPOST Repository | Thu Aug 01 00:00:00 BST 2019

REPOSITORIES: jPOST

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Dataset's files

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			Action	DRS
	M22_MSMS_865.4363_3.t2d	Other
	M22_MSMS_970.4727_4.t2d	Other
	N1_MSMS_1515.7388_4.t2d	Other
	N1_MSMS_2235.0227_5.t2d	Other
	N1_MSMS_2333.0801_7.t2d	Other

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Publications

Molecular basis of egg coat cross-linking sheds light on ZP1-associated female infertility.

Nishimura Kaoru K Dioguardi Elisa E Nishio Shunsuke S Villa Alessandra A Han Ling L Matsuda Tsukasa T Jovine Luca L

Nature communications 20190712 1

Mammalian fertilisation begins when sperm interacts with the egg zona pellucida (ZP), whose ZP1 subunit is important for fertility by covalently cross-linking ZP filaments into a three-dimensional matrix. Like ZP4, a structurally-related component absent in the mouse, ZP1 is predicted to contain an N-terminal ZP-N domain of unknown function. Here we report a characterisation of ZP1 proteins carrying mutations from infertile patients, which suggests that, in human, filament cross-linking by ZP1 i ...[more]

PMID: 31300655

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