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Arabidopsis mitochondrial redox proteomes in different metabolic states

ABSTRACT: Intact mitochondria purified from Arabidopsis thaliana Col-0 seedlings were differentially labeled with iodoTMTs in the presence of different metabolic substrates. Redox proteomes were analyzed in a substrate depleted state and after addition of citrate and 2-oxoglutarate, providing the oxidation states of individual Cys-peptides.

ORGANISM(S): Arabidopsis Thaliana (mouse-ear Cress)

SUBMITTER: Markus Schwarzländer

PROVIDER: PXD014302 | JPOST Repository | Fri Jun 19 00:00:00 BST 2020

REPOSITORIES: jPOST

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			Action	DRS
	150616_V3_P5_RN_FH_HCD_180min_iTMT_2OG_III_8uL_1.raw	Raw
	150616_V3_P5_RN_FH_HCD_180min_iTMT_2OG_III_8uL_2.raw	Raw
	150616_V3_P5_RN_FH_HCD_180min_iTMT_2OG_II_8uL_1.raw	Raw
	150616_V3_P5_RN_FH_HCD_180min_iTMT_2OG_II_8uL_2.raw	Raw
	150616_V3_P5_RN_FH_HCD_180min_iTMT_2OG_I_8uL_1.raw	Raw

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Project description:The aim of this study was to analyze the impact of autotetraploidy on gene expression in Arabidopsis thaliana by comparing diploid versus tetraploid transcriptomes. In particular, this included the comparison of the transcriptome of different tetraploid A. thaliana ecotypes (Col-0 vs. Ler-0). The study was extended to address further aspects. One was the comparison of the transcriptomes in subsequent generations. This intended to obtain information on the genome wide stability of autotetraploid gene expression. Another line of work compared the transcriptomes of different diploid vs. tetraploid tissues. This aimed to investigate whether particular gene groups are specifically affected during the development of A. thaliana autotetraploids. Samples 1-8: Arabidopsis thaliana Col-0 tetraploid transcriptome. Transcriptional profiling and comparison of diploid vs. tetraploid Col-0 seedlings. The experiment was carried out with pedigree of independently generated and assessed tetraploid Col-0 lines. Samples 9-12: Arabidopsis thaliana Ler-0 tetraploid transcriptome. Transcriptional profiling and comparison of diploid vs. tetraploid Ler-0 seedlings. The experiment was carried out with pedigree of independently generated and assessed tetraploid Ler-0 lines. Samples 13-24: Arabidopsis thaliana Col-0 tetraploid transcriptome. Transcriptional profiling and comparison of diploid vs. tetraploid Col-0 leaves (6th - 8th). The experiment was carried out with pedigree of independently generated and assessed tetraploid Col-0 lines. Samples 25-32: Arabidopsis thaliana Ler-0 tetraploid transcriptome. Transcriptional profiling and comparison of diploid vs. tetraploid Ler-0 leaves (6th - 8th). The experiment was carried out with pedigree of independently generated and assessed tetraploid Ler-0 lines. Samples 33-36: Arabidopsis thaliana Ler-0 tetraploid transcriptome. Transcriptional profiling and comparison of tetraploid vs. tetraploid Ler-0 seedlings from the second (F2) and third (F3) generation after induction, respectively. The experiment was carried out with pedigree of independently generated and assessed tetraploid Ler-0 lines. Samples 37-40: Arabidopsis thaliana Col-0 tetraploid transcriptome. Transcriptional profiling and comparison of tetraploid vs. tetraploid Col-0 seedlings from the second (F2) and third (F3) generation after induction, respectively. The experiment was carried out with pedigree of independently generated and assessed tetraploid Col-0 lines. Samples 41-44: Arabidopsis thaliana Col-0/Ler-0 diploid transcriptome. Transcriptional profiling and comparison of diploid Col-0 vs. diploid Ler-0 seedlings. The experiment was carried out with pedigree of esrablished lines. Samples 45-48: Arabidopsis thaliana Col-0/Ler-0 tetraploid transcriptome. Transcriptional profiling and comparison of tetraploid Col-0 vs tetraploid Ler-0 seedlings. The experiment was carried out with pedigree of independently generated and assessed tetraploid Col-0 and Ler-0 lines.

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Arabidopsis mitochondrial redox proteomes in different metabolic states

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