Proteome analysis of human serum and plasma under the different pre-analytical processing
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ABSTRACT:
ORGANISM(S): Homo Sapiens (human)
SUBMITTER: Sumio Ohtsuki
PROVIDER: PXD014305 | JPOST Repository | Mon Mar 23 00:00:00 GMT 2020
REPOSITORIES: jPOST
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Effects of differences in pre-analytical processing on blood protein profiles determined with SWATH-MS.
Nambu Madoka M Nishiumi Shin S Kobayashi Takashi T Masuda Takeshi T Ito Shingo S Yoshida Masaru M Ohtsuki Sumio S
Journal of proteomics 20200521
The purpose of the present study was to assess the differences between the human serum and plasma proteomes, and the stability of human plasma proteins under different storage conditions following blood collection, by means of SWATH-MS analysis. When we compared plasma and serum prepared immediately after blood sampling, 95.5% of 176 quantified proteins differed by less than 1.5-fold. When we compared plasma samples prepared by centrifugation after storage of blood at room temperature for 0, 15 ...[more]
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