Proteomics

Dataset Information

0

An extended PAXT connection is required for turnover of nuclear polyadenylated RNA in human cells

ABSTRACT: Recruitment of the human ribonucleolytic RNA exosome to nuclear polyadenylated (pA+) RNA is facilitated by the Poly(A) Tail eXosome Targeting (PAXT) connection. Besides its core dimer, formed by the exosome co-factor MTR4 and the ZFC3H1 protein, the PAXT connection remains poorly defined. By characterizing nuclear pA+-RNA bound proteomes as well as MTR4-ZFC3H1 containing complexes in conditions favoring PAXT assembly, we here uncover three additional proteins required for PAXT function: ZC3H3, RBM26/RBM27 and the known PAXT-associated protein, PABPN1. The zinc-finger protein ZC3H3 interacts directly with MTR4-ZFC3H1 and loss of either identified PAXT component results in the accumulation of PAXT substrates. Collectively, our results establish new factors involved in the turnover of nuclear pA+ RNA and suggest that these are limiting for PAXT activity.

ORGANISM(S): Homo Sapiens (human)

SUBMITTER: Torben Heick Jensen 

PROVIDER: PXD016070 | JPOST Repository | Thu Oct 31 00:00:00 GMT 2019

REPOSITORIES: jPOST

Json Xml

Dataset's files

Source:
Action DRS
MQ.zip Other
Waldorf_20150617_KI_HS_162-E-tech1.raw Raw
Waldorf_20150617_KI_HS_162-E-tech2.raw Raw
Waldorf_20150617_KI_HS_162-R-tech1.raw Raw
Waldorf_20150617_KI_HS_162-R-tech2.raw Raw
Items per page:
1 - 5 of 5
altmetric image

Publications

The human ZC3H3 and RBM26/27 proteins are critical for PAXT-mediated nuclear RNA decay.

Silla Toomas T   Schmid Manfred M   Dou Yuhui Y   Garland William W   Milek Miha M   Imami Koshi K   Johnsen Dennis D   Polak Patrik P   Andersen Jens S JS   Selbach Matthias M   Landthaler Markus M   Jensen Torben Heick TH  

Nucleic acids research 20200301 5

Recruitment of the human ribonucleolytic RNA exosome to nuclear polyadenylated (pA+) RNA is facilitated by the Poly(A) Tail eXosome Targeting (PAXT) connection. Besides its core dimer, formed by the exosome co-factor MTR4 and the ZFC3H1 protein, the PAXT connection remains poorly defined. By characterizing nuclear pA+-RNA bound proteomes as well as MTR4-ZFC3H1 containing complexes in conditions favoring PAXT assembly, we here uncover three additional proteins required for PAXT function: ZC3H3, R  ...[more]

Similar Datasets

Proteomics JPST000693 An extended PAXT connection is required for turnover of nuclear polyadenylated RNA in human cells [Reanalysis: JPST000693]
2023-03-25 | RPXD034322 | JPOST Repository
Transcriptomics The Human ZC3H3 and RBM26/27 Proteins Are Critical for PAXT-mediated Nuclear RNA Decay
2020-01-09 | GSE131255 | GEO
Other Identification of a nuclear exosome decay pathway for processed transcripts
2016-11-03 | GSE84172 | GEO
Transcriptomics Total poly(A)+ RNA sequencing
2020-06-15 | GSE149187 | GEO
Transcriptomics RNA-seq of Schizosaccharomyces pombe nuclear RNA exosome mutants
2020-04-17 | GSE148799 | GEO
Other Distinct and redundant roles of nuclear RNA exosome targeting complexes
2020-02-06 | GSE137612 | GEO
Transcriptomics The RNA exosome adaptor protein ZFC3H1 functionally competes with nuclear export activity to retain target transcripts
2018-05-14 | GSE108197 | GEO
Transcriptomics Frac-Seq Analysis of ZFC3H1 Knockdown U2OS Cells
2022-03-31 | GSE176144 | GEO
Transcriptomics ZCCHC8 is required for the degradation of pervasive transcripts originating from multiple genomic regulatory features
2021-01-29 | GSE165689 | GEO
Proteomics Rapid factor depletion highlights intricacies of nucleoplasmic RNA degradation
2021-12-23 | PXD026774 | Pride