Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

In vitro TroponinT digestion by calpain and autolysis of calpain.

ABSTRACT: TroponinT was incubated with calpain. Incubation time and labels are described in the sample information. Triplicated LC-MS data were merged and processed with ProteinPilot (ver5).

ORGANISM(S): Homo Sapiens (human) Cellular Organisms

SUBMITTER: Yasuko Ono

PROVIDER: PXD017015 | JPOST Repository | Wed Nov 04 00:00:00 GMT 2020

REPOSITORIES: jPOST

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	A16035_1-4_1uL_125min.mgf	Mgf
	A16035_1-4_1uL_125min.mzid	Mzid
	A16035_1-4_1uL_125min_001.wiff	Wiff
	A16035_1-4_1uL_125min_001.wiff.scan	Wiff
	A16035_1-4_1uL_125min_003.wiff	Wiff

Items per page:

1 - 5 of 20

Publications

Calpain-2 participates in the process of calpain-1 inactivation.

Shinkai-Ouchi Fumiko F Shindo Mayumi M Doi Naoko N Hata Shoji S Ono Yasuko Y

Bioscience reports 20201101 11

Calpain-1 and calpain-2 are highly structurally similar isoforms of calpain. The calpains, a family of intracellular cysteine proteases, cleave their substrates at specific sites, thus modifying their properties such as function or activity. These isoforms have long been considered to function in a redundant or complementary manner, as they are both ubiquitously expressed and activated in a Ca2+- dependent manner. However, studies using isoform-specific knockout and knockdown strategies revealed ...[more]

PMID: 33078830

Similar Datasets

TGF-beta and TLR4-induced transcriptional response

Project description:To assess the pattern of TGF-beta and TLR4-induced gene expression changes at the genome-wide level, confluent healthy skin fibroblasts were incubated with the classic TLR4 ligand, LPS, TGF-beta or transfected with CD4TLR4. At the end of incubation, total RNA was harvested and processed for hybridizations to Illumina Human HT-12V4 microarray chips.

2016-04-23 | GSE79621 | GEO

Transcriptome of the chicken chorioallantoic membrane from male and female embryos, after 11 and 15 days of incubation

Project description:The aim the the sequencing project was to investigate differentially expressed genes between fertilized eggs incubated during 11 or 15 days of incubation (EID11 and EID15). At each stage (EID11 and EID15), chorioallantoic membranes from 10 male embryos and 10 females embryos were collected and processed for RNA extraction and RNAseq analysis (n=40 in total). Thus, the design of the protocol also allowed comparison of differentially expressed genes in the chorioallantoic membrane between male and female embryos

2023-01-20 | GSE199780 | GEO

Arabidopsis thaliana ecotype Columbia [Col-0]): incubated with yeast (saccharomyces cerevisiae) vs. Control

Project description:Transcriptional profiling of Arabidopsis incubated with yeast comparing to control (without yeast incubation).

2017-12-31 | GSE27006 | GEO

Early RNA sequencing of human monocytes in presence of Candida albicans

Project description:C.albicans induces the upregulation of inflammation related genes at the same time it also induces TGF-ß signalling pathway related genes from human blood derived monocytes. RNA sequencing was prerformed from Candida albicans co-incubated monoyctes from 3 different donors. Candida albicans significantly upregulates 6363 genes in human blood derived monocytes in 1h of co-incubation.

2019-10-11 | GSE138681 | GEO

Genome-wide transcript profiling associated with metabolic regulation of Poplar N storage and cycling

Project description:Previous research has shown that glutamine and sucrose treatment of excised poplar stems induces bark storage protein (BSP) gene expression. The objective of this research is to identify changes in gene expression associated with metabolic regulation of nitrogen storage and cycling and use this information to identify potential regulatory genes. Significant, differentially expressed genes were identified in excised poplar stems incubated in solutions of glutamine, sucrose, glycine, glutamine+glucose, and glutamine+sucrose compared to incubation in a water control.

2011-07-01 | GSE29303 | GEO

Lignocellulose-rich residues in anaerobic digestion

Project description:In this study, a complex microbial community from a semi-continues reactor, which only substrate is wheat straw, was incubated in a batch experiment with 13C-cellulose. protein stable isotope probing (protein-SIP) was used to identify the organisms, at high taxonomic resolution, involved in the degradation of cellulose by tracking the incorporation of 13C in the newly synthetized proteins. Thereby providing information regarding identity and function simultaneously and enable the optimization of biotechnologies for biofuels production.

2023-11-30 | PXD036712 | Pride

Updated in-gel digestion for protein analysis by mass spectrometry

Project description:The in-gel digestion of proteins for analysis by liquid chromatograph mass spectrometry has been used since the early 1990s. Although several improvements have contributed to increasing the quality of the data obtained, many recent publications still use sub-optimal approaches. We present an updated in-gel digestion protocol. We show that alternative reducing, alkylating agents and tryptic digestion buffers increase peptide and protein identification and reduce incubation times. Our results indicate that a simultaneous and short, high temperature reduction and alkylation reaction using Tris(2-carboxyethyl)phosphine hydrochloride (TCEP) and chloroacetamide (CAA) with a subsequent gel wash improve protein identification and sequence coverage, diminish peptide side reactions. Additionally, use of 4-(2-Hydroxyethyl)piperazine-1-ethanesulfonic acid buffer (HEPES) allows a significant reduction in the digestion time improving trypsin performance and increasing the peptide recovery. The updated in-gel digestion protocol described here is highly efficient and offers flexibility to be incorporated in any proteomic laboratory.

2018-10-02 | PXD009600 | Pride

Phenological transcriptomic profile of double-crested cormorants during embryonic development

Project description:In this study, we tracked the phenology of the transcriptome during double-crested cormorant (Nannopterum auritum) embryogenesis. Fresh eggs were collected from a reference site and artificially incubated from collection until four days prior to hatching. Embryos were periodically sampled throughout incubation for a total of seven time points. A custom microarray was designed for cormorants (over 14,000 probes) and used for transcriptome analysis in whole body (days 5, 8) and liver tissue (days 12, 14, 16, 20, 24). Three main development periods (early, mid, and late incubation) were identified with differentially expressed genes, gene sets, and pathways within and between each developmental transition.

2022-12-31 | GSE204670 | GEO

Broad and largely overlapping molecular changes arise in thymic and peripheral XCR1+ dendritic cells upon tolerogenic and immunogenic maturation

Project description:This SuperSeries is composed of the SubSeries listed below. DC were isolated from lymphoid organs as previously described (Vremec et al., 2000). DC subsets were sorted by flow cytometry according to the marker combinations described in the 'characteristics: phenotype' field for each sample for each individual series. GeneChip Mouse Gene 1.0 ST (GPL6246) and GeneChip Mouse Genome 430 2.0 (GPL1261) DAT files were processed using Affymetrix Gene Chip Operating System to generate .CEL files. Normalization of the raw Affymetrix expression data with Robust Multi-chip Analysis were performed through Bioconductor in the R statistical environment (version 3.0.2) using the oligo package (GPL6246) and the affy, affyPLM and yaqcaffy packages (GPL1261). For genes symbol with several probeSets, the most variant probSet has been selected. The two platforms where merged by the unique genes symbols. Quantile Normalization was applied on the merged expression arrays to generate Matrix 1. PCA analysis revealed a strong platform effect. Partial Least Square (PLS) was performed to remove the platform effect and yield Matrix2 which was used for subsequent analyses. The data matrices contain log2 values.

2016-08-22 | E-GEOD-71171 | biostudies-arrayexpress

Whole blood transcriptional profiling of healthy volunteers and rheumatoid arthritis patients treated with methotrexate or anti-TNF therapy after ex vivo whole blood stimulation with TNF

Project description:We tested the hypothesis that anti-tumor necrosis factor (TNF) therapy reduces TNF-inducible gene expression in blood. Whole peripheral blood from healthy volunteers and rheumatoid arthritis patients (treated with the monoclonal anti-TNF antibody adalimumab, the soluble TNF receptor etanercept or the standard therapy methotrexate) was incubated at 37 Â°C for three hours in the presence or absence of 10 ng/ml recombinant human TNF. Blood samples were then processed for RNA isolation and transcriptional profiling by gene microarray. This submission includes two human whole genome Agilent Array Designs: A-MEXP-2104 and A-GEOD-20844. Each of the individual raw array files are included as well as a single processed file representing the data matrix of all the merged and normalised data for the probes that are shared by the two array designs.

2017-08-04 | E-MTAB-5094 | biostudies-arrayexpress

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data