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Plasma and crude membrane fraction from mouse liver and kidney

ABSTRACT: The purpose of the present study was to validate the enrichment of transporter proteins in plasma membrane fraction prepared from mouse liver and kidney with Membrane Protein Extraction Kit by means of beads homogenize for efficient subcellular fractionation. The crude and plasma membrane fractions were prepared either with Dounce or beads homogenizers, and protein levels were determined by quantitative proteomics.

ORGANISM(S): Mus Musculus (mouse)

SUBMITTER: Sumio Ohtsuki

PROVIDER: PXD018161 | JPOST Repository | Sat Sep 26 00:00:00 BST 2020

REPOSITORIES: jPOST

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			Action	DRS
	Liver%20CM_Con-1.wiff	Wiff
	Liver%20CM_Con-1.wiff.scan	Wiff
	Liver%20CM_Con-2.wiff	Wiff
	Liver%20CM_Con-2.wiff.scan	Wiff
	Liver%20CM_Con-3.wiff	Wiff

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Publications

Proteomic Evaluation of Plasma Membrane Fraction Prepared from a Mouse Liver and Kidney Using a Bead Homogenizer: Enrichment of Drug-Related Transporter Proteins.

Yagi Ryotaro R Masuda Takeshi T Ogata Seiryo S Mori Ayano A Ito Shingo S Ohtsuki Sumio S

Molecular pharmaceutics 20201002 11

Quantifying the protein levels of drug transporters in plasma membrane fraction helps elucidate the function of these transporters. In this study, we conducted a proteomic evaluation of enriched drug-related transporter proteins in plasma membrane fraction prepared from mouse liver and kidney tissues using the membrane protein extraction kit and a bead homogenizer. Crude and plasma membrane fractions were prepared using either the Dounce or bead homogenizer, and protein levels were determined us ...[more]

PMID: 32902293

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