Site-specific incorporation of two noncanonical amino acids in GFP
Ontology highlight
ABSTRACT:
ORGANISM(S): Mus Musculus (mouse)
SUBMITTER: Simon Elsässer
PROVIDER: PXD019411 | JPOST Repository | Thu Jun 11 00:00:00 BST 2020
REPOSITORIES: jPOST
Dataset's files
| Action | DRS | |||
|---|---|---|---|---|
| 20190218_JEBM_GFP1.raw | Raw | |||
| 20190218_JEBM_GFP2.raw | Raw | |||
| 20190218_JEBM_GFP3.raw | Raw | |||
| 20190218_JEBM_GFP4.raw | Raw | |||
| 20190218_JEBM_GFP5.raw | Raw |
Items per page: 5 1 - 5 of 20 |
Publications
Site-Specific Incorporation of Two ncAAs for Two-Color Bioorthogonal Labeling and Crosslinking of Proteins on Live Mammalian Cells.
Meineke Birthe B Heimgärtner Johannes J Eirich Jürgen J Landreh Michael M Elsässer Simon J SJ
Cell reports 20200601 12
The pyrrolysyl-tRNA/pyrrolysyl-tRNA synthetase (PylT/RS) pair from the archaeon Methanosarcina mazei (Mma) is widely used in protein engineering to site-specifically introduce noncanonical amino acids (ncAAs) through nonsense codon suppression. Here, we engineer the PylT/RS pair encoded by Methanogenic archaeon ISO4-G1 (G1) to be orthogonal to Mma PylT/RS and alter the G1 PylRS active site to accept a complementary ncAA spectrum. We combine the resulting mutual orthogonal pairs for site-specific ...[more]
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