Ontology highlight
ABSTRACT:
ORGANISM(S): Homo Sapiens (human)
SUBMITTER: Yasushi Ishihama
PROVIDER: PXD019664 | JPOST Repository | Fri Jul 16 00:00:00 BST 2021
REPOSITORIES: jPOST
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Niinae Tomoya T Imami Koshi K Sugiyama Naoyuki N Ishihama Yasushi Y
Molecular & cellular proteomics : MCP 20210627
Mass-spectrometry-based phosphoproteomics can identify more than 10,000 phosphorylated sites in a single experiment. But, despite the fact that enormous phosphosite information has been accumulated in public repositories, protein kinase-substrate relationships remain largely unknown. Here, we describe a method to identify endogenous substrates of kinases by using a combination of a proximity-dependent biotin identification method, called BioID, with two other independent methods, kinase-perturbe ...[more]