Dataset Information

Bridgin connects the outer kinetochore to centromeric chromatin

ABSTRACT: In this project, 3XFlag-tagged protein affinity purifications were performed to identify the protein-protein interactors using Cryptococcus neoformans cell lysate. After fractionation with SDS-PAGE, peptide samples for nanoLC-MS/MS were prepared by in-gel digestion. Raw data from Orbotrap Velos pro were processed with MASCOT via Proteome discoverer2.2. Validation of peptide and protein identifications were performed with Scaffold proteome software.

ORGANISM(S): Cryptococcus Neoformans

SUBMITTER: Shreyas Sridhar

PROVIDER: PXD021072 | JPOST Repository | Wed Sep 23 00:00:00 BST 2020

REPOSITORIES: jPOST

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Dataset's files

Source:

			Action	DRS
	SN170794_Wild-type%20control_01.raw	Raw
	SN170795_Wild-type%20control_02.raw	Raw
	SN170796_Wild-type%20control_03.raw	Raw
	SN170797_Wild-type%20control_04.raw	Raw
	SN170798_Wild-type%20control_05.raw	Raw

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Publications

Bridgin connects the outer kinetochore to centromeric chromatin.

Sridhar Shreyas S Hori Tetsuya T Nakagawa Reiko R Fukagawa Tatsuo T Sanyal Kaustuv K

Nature communications 20210108 1

The microtubule-binding outer kinetochore is coupled to centromeric chromatin through CENP-CMif2, CENP-TCnn1, and CENP-UAme1 linker pathways originating from the constitutive centromere associated network (CCAN) of the inner kinetochore. Here, we demonstrate the recurrent loss of most CCAN components, including certain kinetochore linkers during the evolution of the fungal phylum of Basidiomycota. By kinetochore interactome analyses in a model basidiomycete and h ...[more]

PMID: 33420015

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