Proteomics

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Multiple Reaction Monitoring-based Assay for the Targeted Quantitation of Aberrant Substrate-specific Kinase Activity in Non-small Cell Lung Cancer


ABSTRACT: Personalized tissue phosphoproteome was carried to target aberrant signaling in NSCLC from pairs of cancerous tissues and their adjacent normal counterparts from 5 patients. We then report a substrate-specific kinase assay based on multiple reaction monitoring mass spectrometer platform was then developed for relative and absolute quantification of substrate-specific kinase activity at the sensitivity of nanomolar kinase and nanogram cell lysate.

ORGANISM(S): Homo Sapiens (human)

SUBMITTER: Yu-Ju Chen 

PROVIDER: PXD021908 | JPOST Repository | Mon Oct 11 00:00:00 BST 2021

REPOSITORIES: jPOST

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