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Arabidopsis early cold response phosphoproteomics

ABSTRACT: Cold stress is one of the major limiting factors for global crop production. For survival at low temperature, plants need to sense the temperature changes in the surrounding environment. How plants sense and respond to the earliest drop in temperature is still not clearly understood. The plasma membrane and its adjacent extracellular and cytoplasmic sites are the first checkpoints for sensing temperature changes and subsequent events such as signal generation and solute transport. To understand how plants respond to early cold exposure, we have used a mass spectrometry-based phosphoproteomic method to study temporal changes in protein phosphorylation events in Arabidopsis membranes during 5 to 60 min of cold exposure. Phosphoproteomic analysis was carried out by using the following process. Arabidopsis membrane fraction was extracted from leaves. Trypsin digestion and phosphopeptide enrichment by HAMMOC method were performed, then purified peptide sample was analyzed by TripleTOF5600 (AB-SCIEX).

ORGANISM(S): Arabidopsis Thaliana (mouse-ear Cress)

SUBMITTER: Matsuo Uemura

PROVIDER: PXD022275 | JPOST Repository | Tue Nov 02 00:00:00 GMT 2021

REPOSITORIES: jPOST

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			Action	DRS
	P100_Cold_0min-1_1.wiff	Wiff
	P100_Cold_0min-1_1.wiff.scan	Wiff
	P100_Cold_0min-1_2.wiff	Wiff
	P100_Cold_0min-1_2.wiff.scan	Wiff
	P100_Cold_0min-1_3.wiff	Wiff

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Project description:During low temperature exposure, temperate plant species increase their freezing tolerance in a process termed cold acclimation. During deacclimation in response to warm temperatures cold acclimated plants lose freezing tolerance and resume growth and development. While considerable effort has been directed toward understanding the molecular and metabolic basis of cold acclimation, much less information is available about the regulation of deacclimation. Here, we report metabolic (GC-MS) and transcriptional (microarrays, qRT-PCR) responses underlying deacclimation during the first 24 h after a shift of cold acclimated Arabidopsis thaliana (Columbia-0) plants to warm temperature. The data revealed a faster response of the transcriptome than of the metabolome and provided evidence for tightly regulated temporal responses at both levels. Metabolically deacclimation is associated with decreasing contents of sugars, amino acids and glycolytic and TCA cycle intermediates, indicating an increased need for carbon sources and respiratory energy production associated with growth resumption under warm temperature conditions. Deacclimation also involves extensive down-regulation of protein synthesis and changes in the metabolism of lipids and cell wall components. Altered hormonal regulation appears particularly important during deacclimation, with changes in the expression of genes related to auxin, gibberellin, brassinosteroid, jasmonate and ethylene metabolisms. Several transcription factor families controlling fundamental aspects of plant development are significantly regulated during deacclimation, emphasizing that loss of freezing tolerance and growth resumption are interrelated processes that are transcriptionally highly interrelated. Expression patterns of some clock oscillator components during deacclimation resembled those under warm conditions, indicating at least partial re-activation of the circadian clock. This study provide the first comprehensive analysis of the regulation of deacclimation in cold acclimated plants. The data indicate cascades of rapidly regulated genes and metabolites that underly the developmental switch resulting in reduced freezing tolerance and the resumption of growth. They constitute a reference dataset of genes, metabolites and pathways that are crucial during the first rapid phase of deacclimation and will be useful for the further analysis of this important but under-researched plant process. We used whole genome microarrays to monitor changes in gene expression in the Arabidopsis thaliana accession Columbia-0 during the first 24 h after a shift of cold acclimated plants to warm temperature.

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Arabidopsis early cold response phosphoproteomics

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