Identification of Plasmodium falciparum proteins interacting with SURFIN4.1

ABSTRACT: Plasmodium falciparum malaria parasites export several hundred proteins to the cytoplasm of infected red blood cells (RBCs) to modify the cell environment suitable for their growth. A Plasmodium translocon of exported proteins (PTEX) is necessary for both soluble and integral membrane proteins to cross the parasitophorous vacuole membrane surrounding the parasite inside the RBC and an unidentified translocon has been proposed to have a role for integral membrane proteins to cross the parasite plasma membrane. However, the molecular composition of the translocation complex for integral membrane proteins is not well characterized. To gain insights of components of the translocation complex, we compared between tag-fused transgenic parasite lines and their controls. For the first experiment, we used a transgenic line expressing SURFIN4.1N-T-C-TyGFP (mini-SURFIN4.1, v150225v03), consisting of a short N-terminal region, a transmembrane and cytoplasmic regions of an exported integral membrane protein SURFIN4.1, a Ty-tag and GFP, to identify interacting proteins. A wild type parental parasite line (v150226v03) was used as a control. For the second experiment a transgenic line expressing mini-SURFIN4.1 (v160212v06) was compared with a line expressing TyGFP (v160216v01). For the last experiment, we used a transgenic line expressing SURFIN4.1N-T-C-BirA*-HA (v150923v02), consisting of a short N-terminal region, a transmembrane and cytoplasmic regions of an exported integral membrane protein SURFIN4.1, a Bir*A and HA-tag, to identify interacting proteins. In this experiment a transgenic line expressing BirA*-HA (v150923v04) was used as a control.

ORGANISM(S): Cellular Organisms

SUBMITTER: Osamu Kaneko 

PROVIDER: PXD023028 | JPOST Repository | Fri Dec 10 00:00:00 GMT 2021

REPOSITORIES: jPOST