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Peptides with phosphorylation sites found in human DDHD1

ABSTRACT: FLAG-DDHD1-expressing HEK293 or PANC1 cells were treated with okadaic acid, and FLAG-DDHD1 was purified by SDS-PAGE. After the in-gel digestion of purified FLAG-DDHD1 with trypsin or Asp-N, the resultant phosphopeptides were further purified by immobilized metal affinity chromatography (IMAC) or titanium dioxide (TiO2), zirconium dioxide (ZrO2), or TiO2/ZrO2 affinity chromatography. Peptides were analyzed by MALDI-TOF MS/MS. The Mascot analysis using the database of the human Swiss-Prot or FLAG-tagged human DDHD1 sequence revealed more than 10 phosphopeptides of DDHD1 with significant scores.

ORGANISM(S): Homo Sapiens (human)

SUBMITTER: Naoki Matsumoto

PROVIDER: PXD023208 | JPOST Repository | Fri Dec 17 00:00:00 GMT 2021

REPOSITORIES: jPOST

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			Action	DRS
	Ser104,%20139.mztab	Mztab
	Ser104,%20139.raw	Raw
	Ser104,%20139.txt	Txt
	Ser11.mztab	Mztab
	Ser11.raw	Raw

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