Proteomics

Dataset Information

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Proteome-wide analysis of elongating nascent polypeptide chains – IP input


ABSTRACT: Cellular global translation is often measured using ribosome profiling or quantitative mass spectrometry, but these methods do not provide direct information at the level of elongating nascent polypeptide chains (NPCs) and associated co-translational events. We combine quantitative proteomics, pulse labeling with puromycin, and stable isotope-labeled amino acids to analyze thousands of NPCs. Our approach enables global analyses of translational responses and co-translational modifications, providing a framework for dissecting co-translational regulations proteome-wide. The inputs (without IP) were analyzed in triplicates.

ORGANISM(S): Homo Sapiens (human)

SUBMITTER: Yasushi Ishihama 

PROVIDER: PXD024079 | JPOST Repository | Wed Feb 09 00:00:00 GMT 2022

REPOSITORIES: jPOST

Dataset's files

Source:
Action DRS
201018UJ_input1_fr1.raw Raw
201018UJ_input1_fr2.raw Raw
201018UJ_input1_fr4.raw Raw
201018UJ_input1_fr5.raw Raw
201018UJ_input1_fr6.raw Raw
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Publications


Cellular global translation is often measured using ribosome profiling or quantitative mass spectrometry, but these methods do not provide direct information at the level of elongating nascent polypeptide chains (NPCs) and associated co-translational events. Here, we describe pSNAP, a method for proteome-wide profiling of NPCs by affinity enrichment of puromycin- and stable isotope-labeled polypeptides. pSNAP does not require ribosome purification and/or chemical labeling, and captures <i>bona f  ...[more]

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