Dataset Information

In-depth proteome analysis of Hela cell culture conditioned media containing fetal bovine serum.

ABSTRACT: We performed DIA-MS based proteomic analysis of Hela cells conditioned media containing fetal bovine serum by albumin (Alb)-depletion and non-depletion. In addition, we attempted to enrich secreted proteins by reducing the volume of culture medium (10mL, 5mL and 2mL). The supernatants of each culture condition were subjected to albumin depletion followed by DIA-MS. We further analyzed proteins in the conditioned media of HeLa cells with and without tumor necrosis factor (TNF) stimulation.

ORGANISM(S): Homo Sapiens (human)

SUBMITTER: Yusuke Kawashima

PROVIDER: PXD024272 | JPOST Repository | Tue Feb 22 00:00:00 GMT 2022

REPOSITORIES: jPOST

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			Action	DRS
	Alb-depletion_HelaCells_CultureSupernatant_10mL_1.raw	Raw
	Alb-depletion_HelaCells_CultureSupernatant_10mL_2.raw	Raw
	Alb-depletion_HelaCells_CultureSupernatant_10mL_3.raw	Raw
	Alb-depletion_HelaCells_CultureSupernatant_2mL_1.raw	Raw
	Alb-depletion_HelaCells_CultureSupernatant_2mL_2.raw	Raw

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Publications

A Simple Method for In-Depth Proteome Analysis of Mammalian Cell Culture Conditioned Media Containing Fetal Bovine Serum.

Nakamura Ren R Nakajima Daisuke D Sato Hironori H Endo Yusuke Y Ohara Osamu O Kawashima Yusuke Y

International journal of molecular sciences 20210304 5

A conditioned medium of a cell culture is widely used for various biological applications and frequently analyzed to characterize the functional proteins responsible for observed biological functions. However, a large number of abundant proteins in fetal bovine serum (FBS), usually included in the conditioned medium of a mammalian cell culture medium, hampers in-depth proteomic analysis by liquid chromatography-tandem mass spectrometry (LC-MS/MS). For a deep proteomic analysis of a conditioned m ...[more]

PMID: 33806404

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