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Proteome- and N-terminome-driven annotation of proteolysis in beige adipocyte maturation.

ABSTRACT: Beige adipocytes have the reversible thermogenic capacity with increasing Ucp1 expression in response to environmental stimuli. Beige adipocytes acquire thermogenic function during maturation. And their maturation, including changes in morphology and functions, remodeling of the extracellular matrix and organelles turnover, are associated with the proteolytic process. To inspect the post-translational events related to beige adipocyte maturation, we analyze the global proteome and N-terminome to profile proteases and proteolysis products. We induced the differentiation of the preadipocyte cells isolated from the inguinal white adipose tissue into beige adipocytes and harvested the cells every two days. For bottom-up proteome and N-terminome profiling, cell lysates from five time points were subjected to TrypN digestion. N-terminal peptides of the proteolysis products were isolated using the N-terminomics approach based on our TrypN-SCX strategy. In this case, StageTip-based SCX chromatography was used for this large-scale analysis

ORGANISM(S): Mus Musculus (mouse)

SUBMITTER: Yasushi Ishihama

PROVIDER: PXD024334 | JPOST Repository | Mon May 10 00:00:00 BST 2021

REPOSITORIES: jPOST

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			Action	DRS
	181220WAT_0D2_Nt.raw	Raw
	181220WAT_0D3_Nt.raw	Raw
	181220WAT_0D4_Nt.maxq.txt	Txt
	181220WAT_0D4_Nt.raw	Raw
	181220WAT_2D2_Nt.maxq.txt	Txt

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