Proteomics

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Acylated peptide enrichment utilizing lysine deacylases for lysine acylomics

ABSTRACT: Reversible acylation of lysine ε-amino groups, e.g., acetylation, succinylation, maronylation, and myristoylation, is involved in basic physiological processes such as metabolism, cell signaling and aging. In this study, we developed a novel enrichment method for acylated peptides without the use of antibodies, in which endogenously acylated peptides are deacylated by recombinant lysine deacylases based on the enzyme-substrate relationship and enriched by N-hydroxysuccinimidyl chemistry for identification of the acylated sites by nanoscale liquid chromatography-tandem mass spectrometric analysis. To demonstrate the validity of this acylomics platform, we used it to identify acylated sites on chemically acylated model protein samples. We also applied it to the nuclei of HeLa cells to identify endogenous acylated sites.

ORGANISM(S): Homo Sapiens (human)

SUBMITTER: Yasushi Ishihama 

PROVIDER: PXD025845 | JPOST Repository | Thu Jun 03 00:00:00 BST 2021

REPOSITORIES: jPOST

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160909_V8_control.wiff Wiff
160909_V8_control.wiff.scan Wiff
160909_chymotrypsin_control.wiff Wiff
160909_chymotrypsin_control.wiff.scan Wiff
160909_trypsin_control.wiff Wiff
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Acylated peptide enrichment utilizing lysine deacylases for lysine acylomics.

Tsumagari Kazuya K   Ishihama Yasushi Y  

Biochemical and biophysical research communications 20210529

Reversible acylation of lysine ε-amino groups, e.g., acetylation, succinylation, maronylation, and myristoylation, is involved in basic physiological processes such as metabolism, cell signaling and aging. In this study, we developed a novel enrichment method for acylated peptides without the use of antibodies, in which endogenously acylated peptides are deacylated by recombinant lysine deacylases based on the enzyme-substrate relationship and enriched by N-hydroxysuccinimidyl chemistry for iden  ...[more]

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