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Novel roles of small extracellular vesicles in regulating the quiescence and proliferation of neural stem cells

ABSTRACT: We analyzed protein cargos of NSC sEVs from proliferating, resting and reactivating status by proteomics approaches. Our results revealed specific functional clusters of pathways in gene ontology annotations of differentially expressed proteins in three sources of exosomes. Furthermore, we showed the involvement of sEVs in maintaining quiescence, along with entering and exiting the G0 phase by applying an exosome inhibitor. Our findings highlighted the expression level changes of NSCs of sEV protein cargo at different proliferative conditions and further confirmed the involvement of sEVs in entering, maintaining and exiting quiescence of NSCs.

ORGANISM(S): Mus Musculus (mouse)

SUBMITTER: Taeko Kobayashi

PROVIDER: PXD027651 | JPOST Repository | Wed Oct 27 00:00:00 BST 2021

REPOSITORIES: jPOST

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pSNAP: Proteome-wide analysis of elongating nascent polypeptide chains.

Uchiyama Junki J Roy Rohini R Wang Dan Ohtan DO Morikawa Kazuya K Kawahara Yuka Y Iwasaki Mio M Yoshino Chiaki C Mishima Yuichiro Y Ishihama Yasushi Y Imami Koshi K

iScience 20220603 7

Cellular global translation is often measured using ribosome profiling or quantitative mass spectrometry, but these methods do not provide direct information at the level of elongating nascent polypeptide chains (NPCs) and associated co-translational events. Here, we describe pSNAP, a method for proteome-wide profiling of NPCs by affinity enrichment of puromycin- and stable isotope-labeled polypeptides. pSNAP does not require ribosome purification and/or chemical labeling, and captures bona f ...[more]

PMID: 35754732

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