Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Hela digest test as quality control on QE and QE-HF

ABSTRACT: Hela test samples prepared by FASP digestion were run on QE and QE-HF mass spectrometry as quality controls. We tried to compare the protein and peptide identification results between the two machines.

ORGANISM(S): Homo Sapiens (human)

SUBMITTER: Lyu Jiawen

PROVIDER: PXD028625 | JPOST Repository | Sun Sep 18 00:00:00 BST 2022

REPOSITORIES: jPOST

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	HF_20210328_Test_NEWcolumn_Hela_C8_20210328204037.raw	Raw
	HF_20210915_HelaTest_1ug_newC8_6new.raw	Raw
	QE_20210709_Helatest_1.raw	Raw
	QE_C8test_20210910.raw	Raw
	proteinGroups.txt	Txt

Items per page:

1 - 5 of 5

Similar Datasets

A dataset for training/testing machine learning models of peptide feature detection: Human HELA LC-MSMS

Project description:We introduce MSTracer, a tool for peptide feature detection from MS1, which incorporates a machine-learning-combined scoring function based on peptide isotopic distribution and peptide intensity shape on the LC-MS map. By using Support Vector Regression (SVR), the quality of detected peptide features is remarkably improved. By utilising Neural Networks (NN), scores that indicate the quality of features are assigned for detected features as well. We use the Human HELA LC-MSMS dataset to train and test the results and compare with MaxQuant, OpenMS, and Dinosaur.

2021-06-18 | PXD022287 | Pride

COMPARE PT 2016

Project description:In silico Proficiency Test COMPARE

| PRJEB32470 | ENA

Project description:Test run of SRA upload

| PRJNA474771 | ENA

QC mix; quality control mix of 6 compounds trial3

Project description:This is another test of the quality control text mix. This is not really useful for anybody but me.

2023-08-04 | MSV000092593 | MassIVE

Transcriptome profiling of A. thaliana expressing auxin degradation resistant IAA26-P108H-GFP from the IAA26 native promoter

Project description:The goal of this study was to compare the transcriptional profile (RNA-seq) of seven-week-old auxin resistant pIAA26::IAA26-P108H-GFP transgenic plants to non-transformed control plants to identify candidate genes and pathways regulated by IAA26. RNA-seq analysis was performed with A. thaliana ecotype Shahdara seven-week-old leaves from auxin resistant pIAA26::IAA26-P108H-GFP transgenic plants or non-transformed controls. Four fully expanded leaves were collected from each test plant and total RNA from four to six individual test plants was pooled for each of the three biological replicates. Sequencing was carried out on an Illumina HiSeq 1500 platform using rapid run mode with 50 nt single end reads.

2016-04-14 | E-GEOD-75983 | biostudies-arrayexpress

DSS/spermine

Project description:Have you tried spermine?

| PRJEB17622 | ENA

EFSA pilot mock data

Project description:EFSA-ECDC compare pilot submission test repeated data

| PRJEB27692 | ENA

Serum proteomics in a cohort of COVID patients and healthy controls

Project description:This project investigates the serum proteome patterms in recovered COVID patients compared to that in healthy controls. Specifically, serum saamples from 29 recovered patients and 29 age, sex, and race matched healthy controls were collected and subject to DIA-MS proteomics. A fraction of the serum from each sample was pooled for all 58 samples and run as a quality control.

2024-01-03 | PXD036597 | Pride

Sensitivity and protein digestion course of proteomic Filter Aided Sample Preparation

Project description:1. Sensitivity of FASP was tested using SDS lysates from HeLa cells and mouse brain. Peptides were analyzed using a QExactive HF-X instrument. We found that FASP allows effective processing samples at the low and sub-microgram range. 2. Whole cell lysates of Hela cells were processed with FASP using single or double, consecutive or successive, digestion with LysC or trypsin. The generated peptides were analyzed using a LTQ-Orbitrap mass spectrometer. The results show that short digestion times apparently do not affect the number of identified proteins.

2019-11-12 | PXD014288 | Pride

Expression data from livers from mice receiving high-fat (HF) diets supplemented with different berries

Project description:DNA microarray analysis was employed to analyze hepatic gene expression in mice that were protected against HF-induced obesity and liver steatosis (ie HF diets supplemented with lingonberries, blackcurrants or bilberries) and compare to mice who were not protected (HF control) or even experienced increased obesity and fatty liver (HF diet with açai). The study was done to increase understanding of underlying mechanisms of the observed effects.

2015-06-01 | GSE66711 | GEO

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data