Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

EIF4A1-bound 48S complex in HEK293T cells

ABSTRACT: We used HEK293T cells stably expressing eIF4A1 with a 3*FLAG tag at the NH2 terminal. The cells were pull-down assayed by the Sel-TCP-seq method which combines TCP-seq and immunopurification, and the resulting eIFA1-bound 48S complexes were assessed by label-free mass spectrometry.

ORGANISM(S): Homo Sapiens (human)

SUBMITTER: Keiichi I Nakayama

PROVIDER: PXD029301 | JPOST Repository | Sun Oct 24 00:00:00 BST 2021

REPOSITORIES: jPOST

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	orb_201030_A-YK-2_%231-1.raw	Raw
	orb_201030_A-YK-2_%231-10.raw	Raw
	orb_201030_A-YK-2_%231-2.raw	Raw
	orb_201030_A-YK-2_%231-3.raw	Raw
	orb_201030_A-YK-2_%231-4.raw	Raw

Items per page:

1 - 5 of 20

Similar Datasets

eIF4A1-bound 48S complex in HEK293T cells

Project description:We used HEK293T cells stably expressing eIF4A1 with a 3*FLAG tag at the NH2 terminal. The cells were pull-down assayed by the Sel-TCP-seq method which combines TCP-seq and immunopurification, and the resulting eIFA1-bound 48S complexes were assessed by label-free mass spectrometry.

2021-10-24 | PXD029303 | JPOST Repository

eIF4A1-bound 48S complex in HEK293T cells

2021-10-24 | PXD029302 | JPOST Repository

eIF3 complex components in HEK293T cells

Project description:We assessed the eIF3 complex components in eIF3D-V5 tag knock-in HEK293T cells by label-free mass spectrometry after tag pull-down assay.

2021-05-05 | PXD025794 | JPOST Repository

Identification of SOX2-bound mRNA

Project description:In vitro pull down assay was performed using recombinant GST and GST fusion SOX2 protein to precipitate total RNA from urothelial carcinoma cell line BFTC905 Unbound RNA from both reactions and matrix-associated RNA from SOX2 pull down reaction was extracted using TRIZOL reagent This experiment is to identify mRNA bound by SOX2 under in vitro experimental condition

2011-10-25 | E-GEOD-33207 | biostudies-arrayexpress

RNA-seq of total royal jelly and MRJP-3-bound RNA

Project description:MRJP-3 is an RNA binding protein that binds both double- and single-stranded RNA in vitro. To test whether i) MRJP-3 binds RNA in its natural environment, the royal jelly (RJ) and ii) to characterize its RJ RNA partners; a pull down followed by RNA-seq system was developed. MRJP-3 bound RNA was compared to total RJ RNA extracted from the same hive. To establish the system, we first incubated biotinylated MRJP-3, or biotinylated BSA in RJ and pulled the proteins out with strepavidin coated magnetic beads followed by RNA extraction and bioanalyzer profiling. MRJP-3 pull out was enriched for RNA compare to the BSA and just beads controls. The RNA population pulled down with MRJP-3 has similar profile to the total RJ RNA, further demonstrating the MRJP-3 has no affinity to a certain RNA species.

2018-10-28 | E-MTAB-6732 | biostudies-arrayexpress

Identification of SOX2-bound mRNA

2011-10-26 | GSE33207 | GEO

Next Generation Sequencing Facilitates Quantitative Analysis of RNAs bound by DDX5 and RORgt in cultured T helper 17 cell [ChIRP-Seq]

Project description:Purpose: The goals of this study are to compare RNAs bound by DDX5 and RORgt in cultured T helper 17 cell in wildtype background. Methods: Th17 mRNA profiles of 48hrs in vitro cultured T helper 17 cells from wild-type mice were generated by deep sequencing, using Illumina HighSeq. The sequence reads that passed quality filters were analyzed at the transcript isoform level with two methods: Burrows–Wheeler Aligner (BWA) and TopHat followed by CuffDiff. qRT–PCR validation was performed using SYBR Green assays. Results: Among the 3444 RefSeq non-coding RNAs and 46449 NONCODE non-coding RNAs, 2533 were found to be expressed in Th17 cells (FPKM>1). 210 of them were enriched in DDX5 pull-down and 119 of them were enriched in RORgt pull-down. Conclusions: Our study suggest that a subset of 31 ncRNAs were co-enriched in DDX5 and RORgt pull-down.

2015-12-21 | GSE73912 | GEO

Next Generation Sequencing Facilitates Quantitative Analysis of RNAs bound by DDX5 and RORgt in cultured T helper 17 cell [RIP-seq]

2015-12-21 | GSE70109 | GEO

Arabidopsis thaliana

Project description:Pull-down Proteomic

| PRJNA740174 | ENA

Arabidopsis thaliana

Project description:Pull-down Proteomic

| PRJNA740176 | ENA